製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to CEBP Alpha
  • アプリケーション
    適用あり: IP, WBmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    Recombinant fragment corresponding to Rat CEBP Alpha.
    Database link: NM_012524

  • ポジティブ・コントロール
    • Tested with 3T3-L1 cells.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab15048 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IP Use at an assay dependent concentration.
WB Use a concentration of 2 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 30,43 kDa).

Detects bands of approximately 30 kDa and 43 kDa representing CEBP alpha in mouse 3T3-L1 cells.

ターゲット情報

  • 機能
    C/EBP is a DNA-binding protein that recognizes two different motifs: the CCAAT homology common to many promoters and the enhanced core homology common to many enhancers.
  • 配列類似性
    Belongs to the bZIP family. C/EBP subfamily.
    Contains 1 bZIP domain.
  • 細胞内局在
    Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Apoptotic cysteine protease antibody
    • Apoptotic protease Mch 5 antibody
    • C/EBP alpha antibody
    • C/ebpalpha antibody
    • CAP4 antibody
    • Caspase 8 precursor antibody
    • CBF-A antibody
    • CCAAT Enhancer Binding Protein alpha antibody
    • CCAAT/enhancer binding protein (C/EBP), alpha antibody
    • CCAAT/enhancer-binding protein alpha antibody
    • CEBP antibody
    • CEBP A antibody
    • CEBP alpha antibody
    • Cebpa antibody
    • CEBPA_HUMAN antibody
    • FADD homologous ICE/CED 3 like protease antibody
    • FADD like ICE antibody
    • FLICE antibody
    • ICE like apoptotic protease 5 antibody
    • ICE8 antibody
    • MACH antibody
    • MCH5 antibody
    • MORT1 associated CED 3 homolog antibody
    see all

画像


  • Developed using the ECL technique

    Performed under non-reducing conditions.

    Predicted band size : 30,43 kDa
    Observed band size : 30,43 kDa


    Exposure time : 30 seconds

    This image is courtesy of an anonymous Abreview

    Western blot analysis of mouse differentiated adipocyte (20µg/lane) labeling CEBP Alpha with ab15048 at 1/1000. A HRP-conjugated anti-rabbit IgG polyclonal (1/10000) was used as the secondary antibody.

    See Abreview

  • Anti-CEBP Alpha antibody (ab15048) + 3T3-L1 cell lysate.

    Predicted band size : 30,43 kDa
    Western blot of CEBP alpha using ab15048 on 3T3-L1 cells.

参考文献

This product has been referenced in:
  • Jang MK  et al. Histone H3K9 Demethylase JMJD2B Activates Adipogenesis by Regulating H3K9 Methylation on PPAR? and C/EBPa during Adipogenesis. PLoS One 12:e0168185 (2017). WB . Read more (PubMed: 28060835) »
  • Ptasinska A  et al. Identification of a dynamic core transcriptional network in t(8;21) AML that regulates differentiation block and self-renewal. Cell Rep 8:1974-1988 (2014). WB . Read more (PubMed: 25242324) »

See all 3 Publications for this product

レビューと Q&A

Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Non-reduced Denaturing (12.5%)
Sample
Mouse Cell lysate - other (differentiated adipocyte)
Specification
differentiated adipocyte
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Username

Abcam user community

Verified customer

投稿 Jan 21 2014

Thank you for contacting us. Unfortunately the blocking peptide is not available for purchase.

Please do not hesitate to contact us if you need any more advice or information.

I have received the following feedback from the originator of ab15048: According to the original researcher, the "Rabbit anti-C/EBP alpha antibody was created against c/EBP alpha recombinant protein, derived from nucleotide sequence 466-813 of rat ...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (White andipose tissue)
Specification
White andipose tissue
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Username

Abcam user community

Verified customer

投稿 Oct 31 2005

Thank you for your enquiry and your patience in awaiting a response. Ab15048 is derived from a recombinant protein correlating to the nucleotide sequence 466-813 of rat CEBPA (GenBank number NM_012524). The on line data sheet has now been updated to re...

Read More

Regarding ab15048, the immunogen used to generate this antibody was rat CEBP alpha recombinant protein, derived from the nucleotide sequence 466-813 of rat origin (GenBank number NM_012524). If you have any additional questions, please contact us a...

Read More

Thank you for your enquiry and patience. Yes, it appears that aa 981-1348 is wrong as the CEBP alpha protein is smaller than this. However, I am still waiting to obtain information regarding this from the originator of this antibody. I will contact you...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

登録