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Our Abpromise guarantee covers the use of ab15048 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at an assay dependent concentration.|
|WB||Use a concentration of 2 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 30,43 kDa).
Detects bands of approximately 30 kDa and 43 kDa representing CEBP alpha in mouse 3T3-L1 cells.
This image is courtesy of an anonymous Abreview
Western blot analysis of mouse differentiated adipocyte (20µg/lane) labeling CEBP Alpha with ab15048 at 1/1000. A HRP-conjugated anti-rabbit IgG polyclonal (1/10000) was used as the secondary antibody.