製品の概要

  • 製品名
    Anti-CEBP Alpha antibody [15C8]
    CEBP Alpha 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [15C8] to CEBP Alpha
  • 由来種
    Mouse
  • 特異性
    This antibody may cross react with glutamate--ammonia ligase domain containing 1 protein ((Lengsin) Lens glutamine synthase-like) as it has 8/10 aa identical to the immunogen used to generate ab15047 (this protein is part of the Glutamate synthetase family so its compartmentalization to the cytoplasm) unlike CEDPA which is found in the nucleus.
  • アプリケーション
    適用あり: ICC/IF, WB, IPmore details
  • 種交差性
    交差種: Mouse, Rat, Human
    交差が予測される動物種: Hamster, Cow, Xenopus laevis, Schizosaccharomyces pombe, Zebrafish, Goldfish, Aspergillus nidulans, Neurospora crassa
  • 免疫原

    Synthetic peptide corresponding to Mouse CEBP Alpha aa 1-14.
    Sequence:

    MESADFYEVEPRPP


    (Peptide available as ab39791)

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • エピトープ
    Amino terminal region.
  • ポジティブ･コントロール
    • Transfected hu293T cells.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab15047 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use at an assay dependent concentration.
WB Use a concentration of 2 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa).Can be blocked with Mouse CEBP Alpha peptide (ab39791).
IP Use at an assay dependent concentration.

ターゲット情報

  • 機能
    C/EBP is a DNA-binding protein that recognizes two different motifs: the CCAAT homology common to many promoters and the enhanced core homology common to many enhancers.
  • 配列類似性
    Belongs to the bZIP family. C/EBP subfamily.
    Contains 1 bZIP domain.
  • 細胞内局在
    Nucleus.
  • Information by UniProt
  • 参照データベース
    see all
  • 別名
    • Apoptotic cysteine protease antibody
    • Apoptotic protease Mch 5 antibody
    • C/EBP alpha antibody
    • C/ebpalpha antibody
    • CAP4 antibody
    • Caspase 8 precursor antibody
    • CBF-A antibody
    • CCAAT Enhancer Binding Protein alpha antibody
    • CCAAT/enhancer binding protein (C/EBP), alpha antibody
    • CCAAT/enhancer-binding protein alpha antibody
    • CEBP antibody
    • CEBP A antibody
    • CEBP alpha antibody
    • Cebpa antibody
    • CEBPA_HUMAN antibody
    • FADD homologous ICE/CED 3 like protease antibody
    • FADD like ICE antibody
    • FLICE antibody
    • ICE like apoptotic protease 5 antibody
    • ICE8 antibody
    • MACH antibody
    • MCH5 antibody
    • MORT1 associated CED 3 homolog antibody
    see all

画像

  • Western blot - Anti-CEBP Alpha antibody [15C8] (ab15047)
    Western blot - Anti-CEBP Alpha antibody [15C8] (ab15047)
    Anti-CEBP Alpha antibody [15C8] (ab15047) at 2 µg/ml + 293 cell lysates transfected with CEBP alpha.

    Predicted band size: 45 kDa

参考文献

This product has been referenced in:
  • Meng X  et al. Heterogeneous nuclear ribonucleoprotein A1 interacts with microRNA-34a to promote chondrogenic differentiation of mesenchymal stem cells. Am J Transl Res 9:1774-1782 (2017). WB ; Mouse . Read more (PubMed: 28469782) »
  • Khanna-Gupta A  et al. Up-regulation of Translation Eukaryotic Initiation Factor 4E in Nucleophosmin 1 Haploinsufficient Cells Results in Changes in CCAAT Enhancer-binding Protein a Activity: IMPLICATIONS IN MYELODYSPLASTIC SYNDROME AND ACUTE MYELOID LEUKEMIA. J Biol Chem 287:32728-37 (2012). WB . Read more (PubMed: 22851180) »

See all 2 Publications for this product

ab15047 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

レビューと Q&A

レビューを送る 質問を送る

5 reviews or Q&A

10
Votes: Highest First

The species of the gene that the plasmid contained is human.   I would be very grateful to you if you could replace cepb alpha mouse ab with rabbit cebp alpha ab40764.

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused...

Read More

Thanks for the prompt answer. See my answers below please: 1.What is the species of the transfected CEBP alpha? -Human 293 T cells were transfected with C/ebp alpha plazmid  2.How much protein did you load per lane of the gel? -Approximately 70µg p...

Read More

Thank you for sending the additional details. You mentioned that the human cells were transfected with a plasmid; can you please tell me the species of the gene that the plasmid contained? Was this also human, or some other species, for instance mouse/...

Read More

Recently I've contacted you because of CEBP Alpha antibody work failure.   I attached our standard protocol for WB procedure. Unfortunately we don't have word version, that's why I'm sending out a PDF format. I was using 10 % separating gel and 4 % sta...

Read More

Thank you for sending the protocol and image of your blot. I am sorry to see that you are not obtaining a band that corresponds to CEBP Alpha. I am having some difficulty reading the blot image notations. Can you please answer a few questions, to he...

Read More

Many thanks for your e-mail.Following our conversation yesterday,I checked the expasy site and found the protein that you suggests cross reacts with the CEBP alpha antibody.The size ie 57KD fits perfectly with the size of the band that we see on a wes...

Read More

Many thanks for your feedback, I have added a note on the datasheet following your expert advice and would love to find out if indeed the antibody detected the ligase in your cells. Thank you again for your help, I look forward to hearing from you,

The customer is detecting the expected 45kDa band in THP1 cells but a 50-55kDA band in B and T cell tumours (human), which is not found in a nuclear fraction but in a cytosolic fraction suggesting the antibody detects another protein or that the protei...

Read More

As discussed earlier I enclose the links to the databases that I was looking at to find out if the antibody could be cross reacting with other proteins. The protein Glutamate--ammonia ligase domain containing 1 protein (Lengsin) Lens glutamine synth...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

登録