ab26281 recognizes exclusively CEACAM7 transiently expressed on the cell surface of transfected BOSC23 cells. It can be used to distinguish CEACAM7 from all other CEACAM and probably all pregnancy-specific glycoprotein molecules, namely CEACAM1 (BGP/CD66a), CEACAM3 (CGM1/CD66d), CEACAM4 (CGM7), CEACAM6 (NCA/CD66c), CEACAM7 (CGM2), CEACAM8 (CGM6/CD66b) and PSG1 (CD66f) based on its reactivity pattern with stable and transiently transfected mammalian cells expressing individual CEA family members.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CEACAM7 antibody [BAC2] (ab26281)Image from Zhou J et al., World J Surg Oncol. 2011 Dec 23;9:172. Fig 3.; doi:10.1186/1477-7819-9-172; 23 December 2011, World Journal of Surgical Oncology 2011, 9:172
Immunohistochemical analysis of gastric carcinoma tissue, stainin CEACAM7 with ab26281.
Antigen retrieval was performed by heat mediation in a citrate buffer (pH 6). Sections were blocked with 10% normal goat serum in PBS for 30 minutes followed by incubation overnight at 4°C with the primary antibody (1/100 in 1% BSA). Staining was detected using DAB.
Kobayashi M et al. Carcinoembryonic antigen-related cell adhesion molecules as surrogate markers for EGFR inhibitor sensitivity in human lung adenocarcinoma. Br J Cancer107:1745-53 (2012).
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Zhou J et al. Dynamic expression of CEACAM7 in precursor lesions of gastric carcinoma and its prognostic value in combination with CEA. World J Surg Oncol9:172 (2011).
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