製品の概要

  • 製品名Anti-CEACAM6 antibody
    CEACAM6 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to CEACAM6
  • アプリケーション適用あり: IHC-Fr, WB, IHC-Pmore details
  • 種交差性
    交差種: Human
  • 免疫原

    A region within synthetic peptide: IQNPASANRS DPVTLNVLYG PDGPTISPSK ANYRPGENLN LSCHAASNPP, corresponding to internal sequence amino acids 217-266 of Human CEACAM6

  • ポジティブ・コントロール
    • Jurkat cell lysate and human lung tissue.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファーPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 精製度Protein A purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab56234 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-Fr Use at an assay dependent concentration. PubMed: 20220088
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 42 kDa (predicted molecular weight: 37 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.

ターゲット情報

  • 配列類似性Belongs to the immunoglobulin superfamily. CEA family.
    Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • 細胞内局在Cell membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Carcinoembryonic antigen related cell adhesion molecule 6 antibody
    • Carcinoembryonic antigen related cell adhesion molecule 6 (non specific cross reacting antigen) antibody
    • Carcinoembryonic antigen-related cell adhesion molecule 6 antibody
    • CD 66c antibody
    • CD66c antibody
    • CD66c antigen antibody
    • CEA LIKE PROTEIN antibody
    • CEACAM 6 antibody
    • CEACAM6 antibody
    • CEAL antibody
    • CEAM6_HUMAN antibody
    • MGC93832 antibody
    • NCA antibody
    • Non specific cross reacting antigen antibody
    • Non-specific crossreacting antigen antibody
    • Normal cross reacting antigen antibody
    • Normal cross-reacting antigen antibody
    see all

Anti-CEACAM6 antibody 画像

  • All lanes : Anti-CEACAM6 antibody (ab56234) at 1.25 µg/ml

    Lane 1 : Molecular weight markers
    Lane 2 : Jurkat cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 37 kDa
    Observed band size : 42 kDa (why is the actual band size different from the predicted?)
    Gel concentration: 12%
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung tissue labelling CEACAM6 with ab56234 at 4µg/ml. Arrows indicate positively stained alveolar cells. Magnification 400X.

  • All lanes : Anti-CEACAM6 antibody (ab56234) at 1.25 µg/ml

    Lane 1 : Jurkat cell lysate
    Lane 2 : Jurkat cell lysate with blocking peptide at 1

    Lysates/proteins at 25 µg per lane.


    Predicted band size : 37 kDa

Anti-CEACAM6 antibody (ab56234) 使用論文

This product has been referenced in:
  • Allen CE  et al. Cell-specific gene expression in Langerhans cell histiocytosis lesions reveals a distinct profile compared with epidermal Langerhans cells. J Immunol 184:4557-67 (2010). IHC-Fr ; Human . Read more (PubMed: 20220088) »

See 1 Publication for this product

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Thank you for submitting your review. I hope you have received notification that it has been "published" to the datasheet for ab56234 on our website. I am sorry to hear the antibody did not perform as expected. If you have received this vial of ab56234...

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Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry
Sample Human Cultured Cells (Breast cancer cell line T47D)
Specification Breast cancer cell line T47D
Fixative Methanol
Blocking step Serum as blocking agent for 10 minute(s) · Concentration: 10%
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投稿 Nov 21 2011

Application Immunohistochemistry (Frozen sections)
Sample Human Tissue sections (Human primary breast cancer)
Specification Human primary breast cancer
Fixative Methanol
Blocking step Serum as blocking agent for 10 minute(s) · Concentration: 10% · Temperature: 25°C
Username

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Verified customer

投稿 Nov 21 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"