製品の概要

  • 製品名Anti-CEACAM20 antibody [HT-12D8]
  • 製品の詳細
    Mouse monoclonal [HT-12D8] to CEACAM20
  • 特異性ab59626 does not react with CEACAM1, CEACAM3-8 or CEACAM19-21.
  • アプリケーション適用あり: Flow Cyt, ICC/IF, Competitive ELISAmore details
  • 種交差性
    交差種: Human
  • 免疫原

    cDNA encoding human CEACAM20

  • ポジティブ・コントロール
    • CHO cells. FACS: BOSC23 cells

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • バッファーPreservative: None
    Constituents: PBS, pH 7.2
  • Concentration information loading...
  • 精製度Protein G purified
  • 特記事項(精製)ab59626 was purified by protein G affinity chromatography from cell culture supernatants.
  • ポリ/モノモノクローナル
  • クローン名HT-12D8
  • アイソタイプIgG1
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab59626 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt Use 1.2µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
ICC/IF Use 1µg for 106 cells.
Competitive ELISA 1/200 - 1/400.

ターゲット情報

  • 関連性CEA-related cell adhesion molecule 20 (CEACAM20) belongs to the carcinoembryonic antigen (CEA) gene family. It encodes a putative glycoprotein which is membrane-bound via a transmembrane domain. The CEACAM20 protein contains a single N domain followed by 4 immunoglobulin-like A (A1, A2) and B (B1, B2) domains. Expression of CEACAM20 can be found in tissues of prostate, testis, duodenum and small intestine with highest expression in prostate. The function of CEACAM family members varies widely: they function as cell adhesion molecules, tumor suppressors, regulators of lymphocyte and dendritic cell activation, receptors of Neisseria species and other bacteria. High expression of CEACAM20 in tissue of prostate carcinoma and in prostate carcinoma cell lines suggest that CEACAM20 can be used as a tumor marker.
  • 細胞内局在Membrane; Single-pass type I membrane protein
  • 参照データベース
  • 別名
    • carcinoembryonic antigen related cell adhesion molecule 20 antibody
    • UNQ9366 antibody

Anti-CEACAM20 antibody [HT-12D8] 画像

  • Spectral Confocal Microscopy of CHO cells using ab59626 at 1ug/106 cells. CHO cells were transiently transfected with an expression vector encoding CEACAM20. Binding of HT-12D8 (ab59626) was visualized with a FITC-conjugated secondary antibody (green). Actin filaments are labeled with Alexa Fluor-555 Phalloidin (red). Cell nuclei are stained with DAPI (blue).
  • BOSC23 cells were transiently transfected with expression vectors containing either the cDNA of CEACAM1, CEACAM3-8 or CEACAM19-21. Expression of the constructs was tested with monoclonal antibodies known to recognize the corresponding proteins (CEACAM1, 3, 4, 5 and 6: D14HD11; CEACAM7: BAC2; CEACAM8: Tet2; CEACAM19,21: a-myc; CEACAM20: a-flag; green curves). An irrelevant monoclonal antibody served as a negative control (black curves). For specificity testing, protein G purified HT-12D8 (ab59626) was tested on all CEACAM transfectants. A positive signal was obtained onlywith CEACAM20 transfected cells (red curve).

Anti-CEACAM20 antibody [HT-12D8] (ab59626) 使用論文

ab59626 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"