Anti-CEACAM20 抗体 [HT-12D8] (ab59626)
Key features and details
- Mouse monoclonal [HT-12D8] to CEACAM20
- Suitable for: Flow Cyt, ICC/IF
- Reacts with: Human, Recombinant fragment
- Isotype: IgG1
製品の概要
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製品名
Anti-CEACAM20 antibody [HT-12D8] -
製品の詳細
Mouse monoclonal [HT-12D8] to CEACAM20 -
由来種
Mouse -
特異性
ab59626 does not react with CEACAM1, CEACAM3-8 or CEACAM19-21. -
アプリケーション
適用あり: Flow Cyt, ICC/IFmore details -
種交差性
交差種: Human, Recombinant fragment -
免疫原
Other Immunogen Type corresponding to Human CEACAM20. cDNA encoding human CEACAM20
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ポジティブ・コントロール
- FACS: BOSC23 cells
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
バッファー
pH: 7.20
Constituent: PBS -
Concentration information loading...
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精製度
Protein G purified -
特記事項(精製)
ab59626 was purified by protein G affinity chromatography from cell culture supernatants. -
ポリ/モノ
モノクローナル -
クローン名
HT-12D8 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab59626の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
Flow Cyt |
Use 1.2µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
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ICC/IF |
Use 1µg for 106 cells.
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特記事項 |
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Flow Cyt
Use 1.2µg for 106 cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
ICC/IF
Use 1µg for 106 cells. |
ターゲット情報
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関連性
CEA-related cell adhesion molecule 20 (CEACAM20) belongs to the carcinoembryonic antigen (CEA) gene family. It encodes a putative glycoprotein which is membrane-bound via a transmembrane domain. The CEACAM20 protein contains a single N domain followed by 4 immunoglobulin-like A (A1, A2) and B (B1, B2) domains. Expression of CEACAM20 can be found in tissues of prostate, testis, duodenum and small intestine with highest expression in prostate. The function of CEACAM family members varies widely: they function as cell adhesion molecules, tumor suppressors, regulators of lymphocyte and dendritic cell activation, receptors of Neisseria species and other bacteria. High expression of CEACAM20 in tissue of prostate carcinoma and in prostate carcinoma cell lines suggest that CEACAM20 can be used as a tumor marker. -
細胞内局在
Membrane; Single-pass type I membrane protein -
参照データベース
- Entrez Gene: 125931 Human
- SwissProt: Q6UY09 Human
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別名
- carcinoembryonic antigen related cell adhesion molecule 20 antibody
- UNQ9366 antibody
画像
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Spectral Confocal Microscopy of CHO cells using ab59626 at 1ug/106 cells. CHO cells were transiently transfected with an expression vector encoding CEACAM20. Binding of HT-12D8 (ab59626) was visualized with a FITC-conjugated secondary antibody (green). Actin filaments are labeled with Alexa Fluor-555 Phalloidin (red). Cell nuclei are stained with DAPI (blue).
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BOSC23 cells were transiently transfected with expression vectors containing either the cDNA of CEACAM1, CEACAM3-8 or CEACAM19-21. Expression of the constructs was tested with monoclonal antibodies known to recognize the corresponding proteins (CEACAM1, 3, 4, 5 and 6: D14HD11; CEACAM7: BAC2; CEACAM8: Tet2; CEACAM19,21: a-myc; CEACAM20: a-flag; green curves). An irrelevant monoclonal antibody served as a negative control (black curves). For specificity testing, protein G purified HT-12D8 (ab59626) was tested on all CEACAM transfectants. A positive signal was obtained onlywith CEACAM20 transfected cells (red curve).
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (0)
ab59626 は論文での使用が確認できていません。