製品の概要

  • 製品名
    Anti-CEACAM19 antibody [HY-8H10]
  • 製品の詳細
    Mouse monoclonal [HY-8H10] to CEACAM19
  • 特異性
    This antibody reacts with CEACAM19.
  • アプリケーション
    適用あり: Flow Cyt, Competitive ELISAmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Genetic immunisation with cDNA encoding human CEACAM19.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー
    Preservative: None
    Constituents: PBS, pH 7.2
  • Concentration information loading...
  • 精製度
    Protein G purified
  • ポリ/モノ
    モノクローナル
  • クローン名
    HY-8H10
  • アイソタイプ
    IgG1
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab59717 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt
Competitive ELISA
  • 追加情報
    cELISA: 1/200 - 1/400.
    Flow Cyt: Use 1.2µg for 106 cells.
    SDS-PAGE: Use 2µg.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 関連性
      CEACAM19 belongs to the carcinoembryonic antigen (CEA) gene family. It encodes a putative glycoprotein which is membrane-bound via a transmembrane domain. Like all members of the CEACAM family, the CEACAM19 protein is a single-pass type I membrane protein and consists of a single extracellular N domain. CEACAM19 expression is ubiquitous with highest expression in prostate, uterus, fetal brain, mammary gland, adrenal gland, skeletal muscle, small intestine, and kidney.
    • 細胞内局在
      Cell Membrane; Single-pass type I membrane protein .
    • 参照データベース
    • 別名
      • Carcinoembryonic antigen like 1 antibody
      • Carcinoembryonic antigen related cell adhesion molecule 19 antibody
      • CEA related cell adhesion molecule 19 antibody
      • CEAL1 antibody
      • MGC105097 antibody
      see all

    画像

    • BOSC23 cells were transiently trans-fected with an expression vector encoding either CEACAM19 (red curve) or an irrelevant protein (control transfectant). Binding of ab59717 was detected with a PE conjugated secondary antibody. A positive signal was obtained only with CEACAM19 transfected cells.
    • Spectral Confocal Microscopy of CHO cells using ab59717.

      CHO cells were transiently transfected with an expression vector encoding CEACAM19. Binding of ab59717 was visualized with a FITC-conjugated secondary antibody (green). Actin filaments are labeled with Alexa Fluor-555 Phalloidin (red). Cell nuclei are stained with DAPI (blue).

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    参考文献

    This product has been referenced in:
    • Kobayashi M  et al. Carcinoembryonic antigen-related cell adhesion molecules as surrogate markers for EGFR inhibitor sensitivity in human lung adenocarcinoma. Br J Cancer 107:1745-53 (2012). Read more (PubMed: 23099808) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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