製品の概要

  • 製品名Anti-CEACAM 5 + 6 antibody [MUS]
  • 製品の詳細
    Mouse monoclonal [MUS] to CEACAM 5 + 6
  • 特異性MUS reacts specifically with CEACAM 5 (CEA/CD66e) and CEACAM 6 (NCA/CD66c) transiently expressed on the cell surface of transfected BOSC23 cells as demonstrated by flow cytometry.
  • アプリケーション適用あり: Flow Cyt, ELISA, IHC-Fr, WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Full length native Carcino Embryonic Antigen(partially purified) (Human) from a perchloric acid extract from liver metastases of colonic tumors (Schozel S et al.).

  • 特記事項Antibodies produced from cDNA: Conventional technologies usually either generate antibodies against purified proteins, or against synthetic peptides based on amino acid sequences derived from DNA sequence data. Genetic immunization involves introducing the gene in the form of a cDNA directly into an animal which translates this cDNA into protein thus stimulating an immune response against the foreign protein. Although the synthetic peptide approach is comparable in speed, the quality of antibodies generated by genetic immunization is far superior. This is because the protein is made by the immunized animal, utilzing complex cellular mechanisms that allow it to gain a native conformation. Antibodies are then generated against a native protein, such as is found in the blood or tissues of its host species. Membrane-bound or secreted proteins often create problems for conventional antibody technology because in their native form, they are often modified by glycosylation, or in some cases exist as multiple membrane-spanning proteins that are not soluble following isolation or synthesis in recombinant systems. All of these problems are avoided if the immunized animal makes the protein itself. Antibodies generated by genetic immunization have been shown to have binding affinities to the protein in the sub-nanomolar range, which are approximately 100x higher than conventionally developed antibodies and much higher than single chain antibodies. Results confirm published data for much higher avidity of sera generated by genetic immunization as compared with that gained by immunization with a corresponding recombinant protein.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファーPreservative: None
    Constituents: PBS
  • Concentration information loading...
  • 精製度Protein G purified
  • ポリ/モノモノクローナル
  • クローン名MUS
  • ミエローマunknown
  • アイソタイプIgG1
  • 軽鎖の種類unknown
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab4539 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt
ELISA
IHC-Fr
WB
  • 追加情報ELISA: Use at an assay dependant dilution.
    Flow Cyt: Use at an assay dependant dilution.
    IHC-Fr: Use at an assay dependant dilution.
    WB: Use at an assay dependant dilution. Predicted molecular weight Carcino Embryonic Antigen: 84 kDa. Predicted molecular weight CEACAM 6: 41 kDa

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 関連性CEA-related cell adhesion molecules (CEACAM) belong to the carcinoembryonic antigen (CEA) family. It consists of seven CEACAM (CEACAM 1, CEACAM 3-CEACAM 8) and 11 pregnancy-specific glycoprotein (PSG1-PSG11) members. The CEA family proteins belong to the immunoglobulin (Ig) superfamily and are composed of one Ig variable like (IgV) and a varying number (0-6) of Ig constant-like (IgC) domains. CEACAM molecules are membrane-bound either via a transmembrane domain or a glycosyl phosphatidyl inositol (GPI) anchor. CEACAM molecules are differentially expressed in epithelial cells or in leucocytes. Over-expression of CEA/CEACAM 5 in tumors of epithelial origin is the basis of its wide-spread use as a tumor marker. CEACAM 6 expression is strongly up-regulated already during early stages of adenocarcinoma formation e.g. in colon. The function of CEA family members varies widely: they function as cell adhesion molecules, tumor suppressors, regulators of lymphocyte and dendritic cell activation, receptors of Neisseria species and other bacteria.
    • 細胞内局在Plasma membrane
    • 参照データベース
    • 別名
      • Carcinoembryonic antigen antibody
      • CD66c antibody
      • CD66e antibody
      • CEA antibody
      • CEACAM5 antibody
      • CEACAM6 antibody
      • CEAL antibody
      • NCA antibody
      see all

    Anti-CEACAM 5 + 6 antibody [MUS] (ab4539) 使用論文

    This product has been referenced in:
    • Tsutsumida H  et al. RNA interference suppression of MUC1 reduces the growth rate and metastatic phenotype of human pancreatic cancer cells. Clin Cancer Res 12:2976-87 (2006). Read more (PubMed: 16707592) »

    See 1 Publication for this product

    Product Wall

    Actually, since both CEA (CEACAM5) and NCA (CEACAM6) are glycosylated proteins, the apparent molecular weight depends on the extraction method you used...: NCA: In case of a PCA extraction, NCA is usually detected at a molecular weight of 55 kDa, w...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"