製品の概要

  • 製品名
    Anti-CDYL antibody - ChIP Grade
    CDYL 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to CDYL - ChIP Grade
  • 由来種
    Rabbit
  • アプリケーション
    適用あり: CHIPseq, ChIP, IHC-P, WBmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide derived from residues 300 - 400 of Mouse CDYL.

    Immunogen の所有権に関して

    .

  • ポジティブ･コントロール
    • This antibody gave a positive signal in COS7 cells transfected with Human CDYL (Whole Cell Lysate)

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab5188 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
CHIPseq Use at an assay dependent concentration. PubMed: 22009739
ChIP Use at an assay dependent concentration. PubMed: 22009739
IHC-P 1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB 1/1000. Detects a band of approximately 80 kDa (predicted molecular weight: 66 kDa).

ターゲット情報

  • 機能
    Acts as repressor of transcription (By similarity). Has histone acetyltransferase activity, with a preference for histone H4.
  • 組織特異性
    Ubiquitous. Expressed at moderate levels in all tissues examined.
  • 配列類似性
    Contains 1 chromo domain.
  • 細胞内局在
    Nucleus.
  • Information by UniProt
  • 参照データベース
    see all
  • 別名
    • CDY-like antibody
    • CDY-like autosomal antibody
    • Cdyl antibody
    • CDYL: chromodomain protein Y-like antibody
    • CDYL1 antibody
    • CDYL1_HUMAN antibody
    • chromodomain protein Y chromosome-like 6 antibody
    • Chromodomain protein Y like antibody
    • Chromodomain Y-like protein antibody
    • DKFZP586C1622 antibody
    • MGC131936 antibody
    • OTTHUMP00000015984 antibody
    • OTTHUMP00000211185 antibody
    • OTTHUMP00000211186 antibody
    • OTTHUMP00000211189 antibody
    • testis-specific chromodomain Y-like protein antibody
    • Weakly similar to enoyl-CoA hydratase [H.sapiens] antibody
    see all

画像

  • Western blot - Anti-CDYL antibody - ChIP Grade (ab5188)
    Western blot - Anti-CDYL antibody - ChIP Grade (ab5188)Cecile Lestrat, Saadi Khochbin lab, Grenoble, France
    All lanes : Anti-CDYL antibody - ChIP Grade (ab5188) at 1/1000 dilution

    Lane 1 : COS7 (monkey) cells
    Lane 2 : COS7 cells transfected with HA-CDYL-FLAG plasmid

    Predicted band size: 66 kDa
    Observed band size: 83 kDa (why is the actual band size different from the predicted?)



    Rabbit polyclonal to CDYL (ab5188) at 1/1000.

    Left lane (-) COS7 (monkey) cells.
    Right lane (+) COS7 cells transfected with HA-CDYL-FLAG plasmid.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDYL antibody - ChIP Grade (ab5188)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDYL antibody - ChIP Grade (ab5188)

    Image courtesy of Human Protein Atlas

    ab5188 staining CDYL in human male kidney, showing a distinct and strong staining pattern in tubuli and glomeruli cells. Paraffin embedded human skin tissue was incubated with ab5188 (1/1000 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6.

    ab5188 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines. Further results for this antibody can be found at www.proteinatlas.org

    .
  • Western blot - Anti-CDYL antibody - ChIP Grade (ab5188)
    Western blot - Anti-CDYL antibody - ChIP Grade (ab5188)
    All lanes : Anti-CDYL antibody - ChIP Grade (ab5188) at 1 µg/ml

    Lane 1 : COS7 Untransfected Whole Cell Lysate
    Lane 2 : COS7 CDYL (Human) Transfected Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size: 66 kDa
    Observed band size: 80 kDa (why is the actual band size different from the predicted?)
    Additional bands at: 60 kDa, 65 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 12 minutes

参考文献

This product has been referenced in:
  • Qi C  et al. Coordinated regulation of dendrite arborization by epigenetic factors CDYL and EZH2. J Neurosci 34:4494-508 (2014). Rat . Read more (PubMed: 24671995) »
  • Escamilla-Del-Arenal M  et al. Cdyl, a new partner of the inactive X chromosome and potential reader of H3K27me3 and H3K9me2. Mol Cell Biol 33:5005-20 (2013). WB, ICC/IF . Read more (PubMed: 24144980) »

See all 8 Publications for this product

ab5188 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

レビューと Q&A

レビューを送る 質問を送る

5 reviews or Q&A

10
Votes: Highest First

ChIP abreview for Anti-CDYL antibody - ChIP Grade

 Average
Abreviews
Application
ChIP
Detection step
Real-time PCR
Sample
Rat Cell lysate - nuclear (Hippocampal primary Neurons)
Specification
Hippocampal primary Neurons
Negative control
normal Rabbit IgG
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: Formaldehide 1%
Positive control
BDNF Promoter II
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投稿 Oct 06 2014

Western blot abreview for Anti-CDYL antibody - ChIP Grade

 Good
Abreviews
Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (12)
Sample
Rat Cell lysate - whole cell (Primary hippocampal neurons)
Specification
Primary hippocampal neurons
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Read More
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投稿 Sep 25 2014

Dear Hugh, Thanks for your prompt replay. I want to localize male mice stem cells in female mice tissues. Do you have any other suggestion? If I used this Ab would I be able to differ from the two transcripts? Thanks again. Regards,

Thank you for your reply. Unfortunately, I am not an expert in this field and I have not been able to find any publications relating to the expression profiles in mice stem cells. I am not aware of a particular protein or antibody that would recogn...

Read More

Is it possible to get the peptide sequence for this antibody? And also I would like to know if this protein is specific for the chromosome Y. I am looking for a specific marker to localize male stem cells transplanted into female mice.

Thank you for your enquiry. The peptide sequence used to raise this antibody was a synthetic peptide conjugated to KLH derived from within residues 301-314 of Mouse CDYL, corresponding to sequence TGVTAGKRKFIDDR. In reponse to your question it wi...

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I've ordered the ab5188 antibody. I would like to know if it's ok to block the membrane one hour with TBS+5% non-fat dry milk and incubate the membrane with the primary antibody overnight at 4 degrees ?

Thank you for your enquiry. We would suggest blocking the non-specific binding sited with 5% BSA overnight at 4oC. For further information about Western blot application, please take a look at the Protocol on our website

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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