製品の概要

  • 製品名Anti-CDT1 antibody
    CDT1 一次抗体 製品一覧
  • 製品の詳細
    Goat polyclonal to CDT1
  • アプリケーション適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide:

    ARLAHQTRAEEGL

    by a Cysteine residue linker, corresponding to C terminal amino acids 534-546 of Human CDT1

  • ポジティブ・コントロール
    • Human brain lysate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab77091 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
ELISA
  • 追加情報ELISA: 1/64000 in peptide ELISA assay.
    WB: Use at a concentration of 1 - 3 µg/ml. Detects a band of approximately 63 kDa (predicted molecular weight: 63 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能Cooperates with CDC6 to promote the loading of the mini-chromosome maintenance complex onto chromatin to form the pre-replication complex necessary to initiate DNA replication. Binds DNA in a sequence-, strand-, and conformation-independent manner. Potential oncogene.
    • 配列類似性Belongs to the Cdt1 family.
    • 発生段階Present during G1 and early S phase of the cell cycle. Degraded during the late S, G2, and M phases.
    • ドメインThe PIP-box K+4 motif mediates both the interaction with PCNA and the recuitment of the DCX(DTL) complex: while the PIP-box interacts with PCNA, the presence of the K+4 submotif, recruits the DCX(DTL) complex, leading to its ubiquitination.
    • 翻訳後修飾Ubiquitinated by the DCX(DTL) complex, also named CRL4(CDT2) complex, in response to DNA damage, leading to its degradation. Ubiquitination by the DCX(DTL) complex is necessary to ensure proper cell cycle regulation and is PCNA-dependent: interacts with PCNA via its PIP-box, while the presence of the containing the 'K+4' motif in the PIP box, recruit the DCX(DTL) complex, leading to its degradation. The interaction with GMNN protects it against ubiquitination.
      Phosphorylated by cyclin A-dependent kinases which results in the binding of CDT1 to the F-box protein SKP2 and subsequent degradation. Binding to GMNN is not affected by phosphorylation.
    • 細胞内局在Nucleus.
    • Information by UniProt
    • 参照データベース
    • 別名
      • CDT 1 antibody
      • cdt1 antibody
      • CDT1_HUMAN antibody
      • Chromatin licensing and DNA replication factor 1 antibody
      • DNA replication factor antibody
      • DNA replication factor Cdt1 antibody
      • Double parked antibody
      • Double parked Drosophila homolog of antibody
      • Double parked homolog antibody
      • DUP antibody
      • Retroviral integration site 1 antibody
      • Retroviral integration site 2 antibody
      • Retroviral integration site1 antibody
      • Retroviral integration site2 antibody
      • RIS 2 antibody
      • RIS2 antibody
      see all

    Anti-CDT1 antibody 画像

    • Anti-CDT1 antibody (ab77091) at 1 µg/ml + Human brain lysate at 35 µg
      Developed using the ECL technique

      Predicted band size : 63 kDa
      Observed band size : 63 kDa

    Anti-CDT1 antibody (ab77091) 使用論文

    ab77091 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"