製品の概要

  • 製品名
    Anti-CDKN2A/p19ARF antibody [5-C3]
    CDKN2A/p19ARF 一次抗体 製品一覧
  • 製品の詳細
    Rat monoclonal [5-C3] to CDKN2A/p19ARF
  • アプリケーション
    適用あり: WB, IP, ICC/IF, IHC-FoFrmore details
  • 種交差性
    交差種: Mouse
    非交差種: Hamster, Human
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 50 to the C-terminus of Mouse p19ARF.

  • ポジティブ・コントロール
    • 3T3 cell lystate positive control.
  • 特記事項

    This antibody clone is manufactured by Abcam.

    Alternative versions available:

    Anti-CDKN2A/p19ARF antibody [5-C3] - BSA and Azide free (ab174939)

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab26696 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 19 kDa (predicted molecular weight: 17 kDa).
IP Use at an assay dependent concentration.
ICC/IF 1/1000.
IHC-FoFr Use a concentration of 5 µg/ml.

ターゲット情報

  • 関連性
    The gene for CDK2NA generates several transcripts/proteins which differ from each other in their first exons. Three of these transcripts are generated by alternative splicing (isoform 1 a.k.a p16INK4A, isoform 2 and isoform 3 a.k.a p12), two of which are known to function as inhibitors of CDK4 kinase. One other transcript that is generated from this gene contains an alternate reading frame (ARF), with the first exon located 20kb upstream of the remainder of the gene(isoform 4 a.k.a. p14ARF, p19ARF, ARF). In spite of the structural and some functional differences, all the proteins encoded by the CDKN2A gene are involved in cell cycle G1 control.
  • 細胞内局在
    Nuclear
  • 参照データベース
  • 別名
    • ARF antibody
    • CDKN2A antibody
    • Cyclin dependent kinase inhibitor 2A antibody
    • INK4 antibody
    • MTS1 antibody
    • P19 antibody
    see all

画像

  • All lanes : Anti-CDKN2A/p19ARF antibody [5-C3] (ab26696)

    Lane 1 : Total cell lysate from NIH3T3 cells with Arf gene deleted
    Lane 2 : MEF total cell lysate

    Secondary
    anti-rat IgG-HRP

    Predicted band size : 17 kDa
    Observed band size : 19 kDa (why is the actual band size different from the predicted?)
  • Immunofluorescent detection of p19ARF. MEF cells (1 and 2) or NIH3T3 cells with arf gene deleted (3 and 4) were stained with anti-p19ARF 5C3 followed by a fluorescent-labelled anti-rat IgG (1 and 3) and Hoechst dye to visualise nuclei (2 and 4).
  • Immunoprecipitation using anti-p19ARF 5C3. NIH3T3 cells expressing HA-p19ARF were incubated with a control rat IgG (lane 1) or anti-p19ARF 5C3 (2). Precipitated proteins were analysed by Western blot using anti-HA antibody.

参考文献

This product has been referenced in:
  • Lee CK  et al. Syk-mediated tyrosine phosphorylation of mule promotes TNF-induced JNK activation and cell death. Oncogene 35:1988-95 (2016). Read more (PubMed: 26212014) »
  • Bartesaghi S  et al. Inhibition of oxidative metabolism leads to p53 genetic inactivation and transformation in neural stem cells. Proc Natl Acad Sci U S A 112:1059-64 (2015). WB . Read more (PubMed: 25583481) »

See all 15 Publications for this product

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