Anti-CDKN2A/p16INK4a 抗体 - N-terminal (ab189034)
Key features and details
- Rabbit polyclonal to CDKN2A/p16INK4a - N-terminal
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-CDKN2A/p16INK4a antibody - N-terminal
CDKN2A/p16INK4a 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to CDKN2A/p16INK4a - N-terminal -
由来種
Rabbit -
特異性
Expression levels of the CDKN2A/p16INK4a protein may vary with sample type. It’s barely expressed in normal tissue, and mostly expressed in some tumour tissues, such as cervical cancer, breast cancer and so on. Moreover, only expressed in some cell lines. Please see images for recommended positive controls.
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アプリケーション
適用あり: ICC/IF, WB, IHC-Pmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rat -
免疫原
Synthetic peptide corresponding to Human CDKN2A/p16INK4a (N terminal). 19 amino acid peptide.
Database link: P42771 -
ポジティブ・コントロール
- Human colon tissues. Human colon cells. Mouse colon tissue lysate.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.4
Preservative: 0.02% Sodium azide
Constituent: 99% PBS -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab189034の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC/IF |
Use a concentration of 20 µg/ml.
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WB | (2) |
Use a concentration of 1 - 2 µg/ml.
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IHC-P | (2) |
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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特記事項 |
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ICC/IF
Use a concentration of 20 µg/ml. |
WB
Use a concentration of 1 - 2 µg/ml. |
IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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細胞内局在
Cytoplasmic and Nuclear -
参照データベース
- Entrez Gene: 1029 Human
- Entrez Gene: 12578 Mouse
- Entrez Gene: 25163 Rat
- Omim: 600160 Human
- SwissProt: P42771 Human
- SwissProt: P51480 Mouse
- SwissProt: Q9R0Z3 Rat
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製品の状態
There are 4 isoforms produced by alternative splicing. Isoform 1 also known as: p16INK4a; Isoform 3 also known as: p12; Isoform 4 also known as: p14ARF; p19ARF; ARF. -
別名
- CCM2 antibody
- CDK4 inhibitor p16 INK4 antibody
- CDK4I antibody
see all
画像
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All lanes : Left: ab189034 at 2 ug/mL
Right: ab270058 at 1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : HEK-293 cell lysate
Lane 3 : Saos-2 cell lysate
Lane 4 : MCF7 cell lysate
Lysates/proteins at 20 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Observed band size: 17 kDa why is the actual band size different from the predicted?Western blot: Anti-CDKN2A antibody (ab189034) staining at 2 ug/ml, shown in green; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution, shown in magenta. In Western blot, ab189034 was shown to bind specifically to CDKN2A however we recommend using ab270058 for more specific binding in Western blot. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3% milk in TBS-0.1% Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4°C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue sections labeling CDKN2A/p16INK4a with ab189034 at 5 µg/ml. Heat mediated antigen retrieval was performed.
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Immunofuorescent analysis of human colon cells labeling CDKN2A using ab189034 at 20 µg/ml.
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Immunohistochemical analysis of formalin fixed, paraffin embedded human colon tissue labeling CDKN2A using ab189034 at 5 µg/ml.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (58)
ab189034 は 58 報の論文で使用されています。
- Cui X et al. HMGCL-induced β-hydroxybutyrate production attenuates hepatocellular carcinoma via DPP4-mediated ferroptosis susceptibility. Hepatol Int 17:377-392 (2023). PubMed: 36508088
- Mendes-Silva AP et al. Abnormal expression of cortical cell cycle regulators underlying anxiety and depressive-like behavior in mice exposed to chronic stress. Front Cell Neurosci 16:999303 (2022). PubMed: 36568887
- Fang Y et al. Age-related GSK3β overexpression drives podocyte senescence and glomerular aging. J Clin Invest 132:N/A (2022). PubMed: 35166234
- Murie M et al. ATase inhibition rescues age-associated proteotoxicity of the secretory pathway. Commun Biol 5:173 (2022). PubMed: 35217767
- Kim H et al. Attenuation of intrinsic ageing of the skin via elimination of senescent dermal fibroblasts with senolytic drugs. J Eur Acad Dermatol Venereol 36:1125-1135 (2022). PubMed: 35274377