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RabMAb

Anti-CDKN2A/p16INK4a 抗体 [EPR1473] (ab108349)

製品の概要

  • 製品名
    Anti-CDKN2A/p16INK4a antibody [EPR1473]
    CDKN2A/p16INK4a 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR1473] to CDKN2A/p16INK4a
  • アプリケーション
    適用あり: WB, IP, IHC-P, Flow Cyt, ICC/IFmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human CDKN2A/p16INK4a aa 100 to the C-terminus (C terminal).

  • ポジティブ・コントロール
    • WB: HeLa, HEK293, 293T, and Saos-2 cell lysates. IHC-P: Human cervical carcinoma tissue. ICC/IF: HeLa cells. Flow Cyt: HEK293 and HeLa cells. IP: HeLa cell lysate.
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    A trial size is available to purchase for this antibody.

     

    Alternative versions available:

    Anti-CDKN2A/p16INK4a antibody (Alexa Fluor® 488) [EPR1473] (ab192053)

    Anti-CDKN2A/p16INK4a antibody (Alexa Fluor® 647) [EPR1473] (ab192054)

    Anti-CDKN2A/p16INK4a antibody (HRP) [EPR1473] (ab192080)

    Anti-CDKN2A/p16INK4a antibody (Phycoerythrin) [EPR1473] (ab209579)

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab108349 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/2000. Predicted molecular weight: 17 kDa.

For unpurified use at 1/1000 - 1/10000.

IP 1/30.

For unpurified use at 1/10 - 1/100.

IHC-P 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

See protocols (link: http://www.abcam.com/protocols/ihc-antigen-retrieval-protocol).

For unpurified use at 1/250 - 1/500.

Flow Cyt 1/270.

For unpurified use at 1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

ICC/IF 1/270.

For unpurified use at 1/100 - 1/250.

ターゲット情報

  • 細胞内局在
    Cytoplasmic and Nuclear
  • 参照データベース
  • 製品の状態
    There are 4 isoforms produced by alternative splicing. Isoform 1 also known as: p16INK4a; Isoform 3 also known as: p12; Isoform 4 also known as: p14ARF; p19ARF; ARF.
  • 別名
    • CCM2 antibody
    • CDK4 inhibitor p16 INK4 antibody
    • CDK4I antibody
    • CDKN2 antibody
    • CDKN2A antibody
    • Cell cycle negative regulator beta antibody
    • CMM2 antibody
    • Cyclin dependent kinase 4 inhibitor A antibody
    • Cyclin dependent kinase inhibitor 2A (melanoma p16 inhibits CDK4) antibody
    • Cyclin Dependent Kinase Inhibitor 2A antibody
    • Cyclin dependent kinase inhibitor 2A isoform 4 antibody
    • Cyclin dependent kinase inhibitor 2A isoforms 1/2/3 antibody
    • Cyclin dependent kinase inhibitor p16 antibody
    • INK4 antibody
    • INK4A antibody
    • MLM antibody
    • MTS1 antibody
    • Multiple tumor suppressor 1 antibody
    • p14 antibody
    • p16 antibody
    • P16INK4 antibody
    • p16INK4a antibody
    • p19 antibody
    • p19Arf antibody
    • TP16 antibody
    see all

Anti-CDKN2A/p16INK4a antibody [EPR1473] 画像

  • All lanes : Anti-CDKN2A/p16INK4a antibody [EPR1473] (ab108349) at 1/2000 dilution (purified)

    Lane 1 : HEK293 cell lysate
    Lane 2 : Saos-2 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size : 17 kDa
    Observed band size : 17 kDa

    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cervix carcinoma tissue labelling CDKN2A/p16INK4a with purified ab108349 at 1/100. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

  • Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling CDKN2A/p16INK4a with unpurified ab108349 at a dilution of 1/100.

  • Overlay histogram showing HEK293 cells stained with unpurified ab108349 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab108349, 1/100) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HEK293 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

  • ab108349 (purified) at 1/30 immunoprecipitating CDKN2A/p16INK4a in HeLa cell lysate. For western blotting, a peroxidase-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/1000).

    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

  • Anti-CDKN2A/p16INK4a antibody [EPR1473] (ab108349) at 1/2000 dilution (purified) + HeLa cell lysate at 10 µg

    Secondary
    Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size : 17 kDa
    Observed band size : 17 kDa

    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

  • All lanes : Anti-CDKN2A/p16INK4a antibody [EPR1473] (ab108349) at 1/1000 dilution (unpurified)

    Lane 1 : HeLa cell lysate
    Lane 2 : 293T cell lysate
    Lane 3 : Saos-2 cell lysate

    Lysates/proteins at 10 µg per lane.


    Predicted band size : 17 kDa
  • Flow Cytometry analysis of HEK293 cells labelling CDKN2A/p16INK4a with purified ab108349 at 1/270 (red). Cells were fixed with 2% paraformaldehyde. A FITC-conjugated goat anti-rabbit IgG (1/150) was used as the secondary antibody. Black - Isotype control, rabbit monoclonal IgG. Blue - Unlabelled control, cells without incubation with primary and secondary antibodies.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cervical carcinoma tissue labelling CDKN2A/p16INK4a with unpurified ab108349 at a dilution of 1/250.

Anti-CDKN2A/p16INK4a antibody [EPR1473] (ab108349) 使用論文

This product has been referenced in:
  • Al-Khalaf HH  et al. p16(INK4A) Enhances the Transcriptional and the Apoptotic Functions of p53 through DNA-dependent Interaction. Mol Carcinog N/A:N/A (2017). Read more (PubMed: 28218424) »
  • Laporte AN  et al. HDAC and Proteasome Inhibitors Synergize to Activate Pro-Apoptotic Factors in Synovial Sarcoma. PLoS One 12:e0169407 (2017). WB ; Human . Read more (PubMed: 28056055) »

See all 24 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (vascular smooth muscle celss)
Gel Running Conditions
Reduced Denaturing (10% gel)
Loading amount
30 µg
Specification
vascular smooth muscle celss
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Jul 21 2017

Application
Western blot
Sample
Human Cell lysate - nuclear (Vascular smooth muscle cells)
Gel Running Conditions
Reduced Denaturing
Loading amount
30 µg
Specification
Vascular smooth muscle cells
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Abcam user community

Verified customer

投稿 Jul 04 2017

Application
ELISA
Sample
Human Recombinant protein (Purified recombinant p16 (overexpressed in HEK293))
Specification
Purified recombinant p16 (overexpressed in HEK293)
Type
Sandwich (Detection)
Blocking step
SuperBlock PBS Pierce #37580 as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10µg/mL · Temperature: 20°C
Username

Abcam user community

Verified customer

投稿 Apr 28 2014

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (B16F10 melanoma cancer implanted)
Specification
B16F10 melanoma cancer implanted
Fixative
4% Formalin
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: autoclaved for 10min at 121 Celcius (10mM Sodium Citrate, pH 6.0)
Permeabilization
Yes - 0.1% SDS
Blocking step
BSA as blocking agent for 15 minute(s) · Concentration: 1% · Temperature: 25°C
Username

Ms. Seontae Kim

Verified customer

投稿 Mar 15 2013

Thank you for contacting us.

We do not provide Abtrial code for tested species and applications. Please check and explain this to customer;

http://www.abcam.com/index.html?pageconfig=resource&rid=11998&viapagetrap=collaboratio...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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