製品の概要

  • 製品名Anti-CDKN2A/p16INK4a antibody [EP4353Y(3)]
    CDKN2A/p16INK4a 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EP4353Y(3)] to CDKN2A/p16INK4a
  • アプリケーション適用あり: WB, ICC/IF, Flow Cytmore details
    適用なし: IHC-P or IP
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant full length Human CDKN2A/p16INK4a tagged

  • ポジティブ・コントロール
    • HeLa cells and 293 cell lysate
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

     

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    A trial size is available to purchase for this antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Alternative versions available:

    Anti-CDKN2A/p16INK4a antibody (Alexa Fluor® 488) [EP4353Y(3)] (ab199756)

    Anti-CDKN2A/p16INK4a antibody (Alexa Fluor® 647) [EP4353Y(3)] (ab199819)

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab81278 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/2000. Predicted molecular weight: 17 kDa.
ICC/IF 1/100 - 1/500.
Flow Cyt 1/50 - 1/100. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
  • 追加情報Is unsuitable for IHC-P or IP.
  • ターゲット情報

    • 細胞内局在Cytoplasmic and Nuclear
    • 参照データベース
    • 製品の状態There are 4 isoforms produced by alternative splicing. Isoform 1 also known as: p16INK4a; Isoform 3 also known as: p12; Isoform 4 also known as: p14ARF; p19ARF; ARF.
    • 別名
      • CCM2 antibody
      • CDK4 inhibitor p16 INK4 antibody
      • CDK4I antibody
      • CDKN2 antibody
      • CDKN2A antibody
      • Cell cycle negative regulator beta antibody
      • CMM2 antibody
      • Cyclin dependent kinase 4 inhibitor A antibody
      • Cyclin dependent kinase inhibitor 2A (melanoma p16 inhibits CDK4) antibody
      • Cyclin Dependent Kinase Inhibitor 2A antibody
      • Cyclin dependent kinase inhibitor 2A isoform 4 antibody
      • Cyclin dependent kinase inhibitor 2A isoforms 1/2/3 antibody
      • Cyclin dependent kinase inhibitor p16 antibody
      • INK4 antibody
      • INK4A antibody
      • MLM antibody
      • MTS1 antibody
      • Multiple tumor suppressor 1 antibody
      • p14 antibody
      • p16 antibody
      • P16INK4 antibody
      • p16INK4a antibody
      • p19 antibody
      • p19Arf antibody
      • TP16 antibody
      see all

    Anti-CDKN2A/p16INK4a antibody [EP4353Y(3)] 画像

    • All lanes : Anti-CDKN2A/p16INK4a antibody [EP4353Y(3)] (ab81278) at 1/20000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : 293 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti rabbit at 1/2000 dilution

      Predicted band size : 17 kDa
      Observed band size : 17 kDa
    • Immunofluorescent staining of HeLa cells using 1/100 ab81278
    • Overlay histogram showing HEK293 cells stained with ab81278 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab81278, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HEK293 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

    Anti-CDKN2A/p16INK4a antibody [EP4353Y(3)] (ab81278) 使用論文

    This product has been referenced in:
    • Tsuno A  et al. Fasudil inhibits the proliferation and contractility and induces cell cycle arrest and apoptosis of human endometriotic stromal cells: a promising agent for the treatment of endometriosis. J Clin Endocrinol Metab 96:E1944-52 (2011). Read more (PubMed: 21917869) »
    • Kawano Y  et al. Application of the histone deacetylase inhibitors for the treatment of endometriosis: histone modifications as pathogenesis and novel therapeutic target. Hum Reprod 26:2486-98 (2011). WB ; Human . Read more (PubMed: 21715447) »

    See all 2 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"