Anti-CDKN2A/p16INK4a 抗体 [2D9A12] (ab54210)

製品の概要

  • 製品名Anti-CDKN2A/p16INK4a antibody [2D9A12]
    CDKN2A/p16INK4a 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [2D9A12] to CDKN2A/p16INK4a
  • アプリケーション適用あり: Flow Cyt, ELISA, IHC-Pmore details
    適用なし: WB
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Recombinant fragment corresponding to Human CDKN2A/p16INK4a.
    Database link: P42771

  • ポジティブ・コントロール
    • Rat liver tissue; Human brain tissue; Human brain tumor tissue; PFA/Tween HeLa cells.
  • 特記事項

    We have received positive and negative feedback from customers for ICC/IF. ICC/IF is not a batch-tested application for this antibody and we are not able to cover it under our Abpromise guarantee.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab54210 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt 1/50. ab170192-Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
ELISA 1/10000.
IHC-P 1/200 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Recommended antigen retrieval: Place slides in a special glass slide holder and fill in the rack with slides to ensure even heating. Rinse rack in 1L of Citrate buffer in ordinary pressure cooker,(0.01M,pH6.0) to immerse all slides. Cover the cap and heat for 15-20 minutes on electric cooker until water boils. Begin timing, only 2 minutes in high-pressure Remove the pressure cooker, Allow it to cool(Cool the slides at room temperature) Rinse the slides in PBS for 5 minutes
  • 追加情報Is unsuitable for WB.
  • ターゲット情報

    • 細胞内局在Cytoplasmic and Nuclear
    • 参照データベース
    • 製品の状態There are 4 isoforms produced by alternative splicing. Isoform 1 also known as: p16INK4a; Isoform 3 also known as: p12; Isoform 4 also known as: p14ARF; p19ARF; ARF.
    • 別名
      • CCM2 antibody
      • CDK4 inhibitor p16 INK4 antibody
      • CDK4I antibody
      • CDKN2 antibody
      • CDKN2A antibody
      • Cell cycle negative regulator beta antibody
      • CMM2 antibody
      • Cyclin dependent kinase 4 inhibitor A antibody
      • Cyclin dependent kinase inhibitor 2A (melanoma p16 inhibits CDK4) antibody
      • Cyclin Dependent Kinase Inhibitor 2A antibody
      • Cyclin dependent kinase inhibitor 2A isoform 4 antibody
      • Cyclin dependent kinase inhibitor 2A isoforms 1/2/3 antibody
      • Cyclin dependent kinase inhibitor p16 antibody
      • INK4 antibody
      • INK4A antibody
      • MLM antibody
      • MTS1 antibody
      • Multiple tumor suppressor 1 antibody
      • p14 antibody
      • p16 antibody
      • P16INK4 antibody
      • p16INK4a antibody
      • p19 antibody
      • p19Arf antibody
      • TP16 antibody
      see all

    Anti-CDKN2A/p16INK4a antibody [2D9A12] 画像

    •  ab54210 (1:500) staining p16 INK4a in human brain tumor tissue by immunohistochemistry using paraffin embedded tissue. Nuclear staining (by DAB staining) is shown.

    • ab54210 staining CDKN2A/p16INK4a in Human cervix and ovarian carcinoma tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/4000 in Dako diluent) for 20 minutes at 25°C. An undiluted HRP-conjugated Mouse polymer was used as the secondary antibody.

      See Abreview

    • ab54210 staining CDKN2A/p16INK4a in Human skin tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 2% BSA for 1 hour at 22°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/100 in 1% NGS/TBS) for 20 hours at 4°C. Ab6785 (1/800) was used as the secondary antibody.

      See Abreview

    • ab54210 (1:500) staining p16 INK4a in rat liver tissue by immunohistochemistry using paraffin embedded tissue. Nuclear staining (by DAB staining) is shown.

    • Overlay histogram showing HeLa cells stained with ab54210 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab54210, 1/50 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde/permeabilized in 0.1% PBS-Tween used under the same conditions.

    •  ab54210 (1:500) staining p16 INK4a in human brain tissue by immunohistochemistry using paraffin embedded tissue. Nuclear staining (by DAB staining) is shown.

    Anti-CDKN2A/p16INK4a antibody [2D9A12] (ab54210) 使用論文

    This product has been referenced in:
    • Hsu CK  et al. Bowen's disease with features resembling myrmecia wart. J Dermatol 42:90-3 (2015). Read more (PubMed: 25387725) »
    • Maeda M  et al. Intermittent high glucose implements stress-induced senescence in human vascular endothelial cells: role of superoxide production by NADPH oxidase. PLoS One 10:e0123169 (2015). Human . Read more (PubMed: 25879533) »

    See all 14 Publications for this product

    Product Wall

    Application Western blot
    Sample Mouse Tissue lysate - whole (Inguinal Adipose Tissue)
    Gel Running Conditions Reduced Denaturing (2-20% Precast Gel, 100V for 1 hr 20 min to run)
    Loading amount 40 µg
    Specification Inguinal Adipose Tissue
    Blocking step SuperBlock T20 (TBS) Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 23°C
    Username

    Abcam user community

    Verified customer

    投稿 Sep 13 2016

    Application Immunohistochemistry (Frozen sections)
    Sample Mouse Tissue sections (Brain (medulloblastoma preneoplastic lesions))
    Permeabilization Yes - 0.3% Triton
    Specification Brain (medulloblastoma preneoplastic lesions)
    Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C
    Fixative Paraformaldehyde
    Username

    Lukas Tamayo

    Verified customer

    投稿 Jun 20 2016

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunocytochemistry/ Immunofluorescence
    Sample Mouse Cell (brain section)
    Permeabilization Yes - 0.8% Triton-100 in PBS
    Specification brain section
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
    Fixative Paraformaldehyde
    Username

    Peisu Zhang

    Verified customer

    投稿 Mar 15 2016

    Application Immunocytochemistry/ Immunofluorescence
    Sample Human Cell (vascular smooth muscle cell)
    Permeabilization Yes - NP40
    Specification vascular smooth muscle cell
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
    Fixative Formaldehyde
    Username

    Abcam user community

    Verified customer

    投稿 Jan 06 2016

    Application Western blot
    Loading amount 25 µg
    Gel Running Conditions Reduced Denaturing (15%gel)
    Sample Mouse Tissue lysate - whole (hippocampus)
    Specification hippocampus
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    投稿 Apr 10 2015



    CDKN2A/p16INK4a est une protéine qui est exprimé dans 63% de tumeurs et non pas que dans les tumeurs cérébrales. Vous pouvez donc également utiliser d’autres tissus cancéreux comme contrôl...

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    Thank you for contacting us.

    Human brain slides, such as ab4304, or humor brain tumor slides such as ab4679 should be good positive controls.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need ...

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    Thank you for contacting us.

    Here are the links to the Human Protein Atlas, including ones to the sites for normal and cancer tissue as well as cell lines. Several brain cell lines are listed as expressing CDKN2A in moderate levels.

    h...

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    Thank you for contacting Abcam and it was good to meet you at the Distributor Day events.

    I have looked through our catalogue to try and find some antibodies that would match your customers needs and I have placed links to the best matches bel...

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    Thank you for your enquiry and your interest in our products.
    We have several antibodies against CDKN2A but none of them have been tested for cross-reactivity with Syrian hamster (Mesocricetus auratus).
    Regarding ab54210 - the exact immunogen...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"