製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to CDKN2AIP
  • アプリケーション
    適用あり: ICC/IF, IHC-P, WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    antigen sequence: ELRCKSVYLG TGCGKSKENA KAVASREALK LFLKKKVVVK ICKRKYRGSE IEDLVLLDEE SRPVNLPPAL, corresponding to amino acids of Human CDKN2AIP.

  • ポジティブ・コントロール
    • Human testis tissue; RT 4, U 251 MG, Human plasma and liver lysates.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab122715 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/250 - 1/500.

ターゲット情報

  • 機能
    Activates p53/TP53 by CDKN2A-dependent and CDKN2A-independent pathways.
  • 組織特異性
    Ubiquitously expressed.
  • 配列類似性
    Belongs to the CARF family.
    Contains 1 DRBM (double-stranded RNA-binding) domain.
  • 翻訳後修飾
    May be ubiquitinated.
  • 細胞内局在
    Nucleus > nucleoplasm.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Calcium response factor antibody
    • CARF antibody
    • CARF_HUMAN antibody
    • CDKN2A interacting protein antibody
    • CDKN2A-interacting protein antibody
    • Cdkn2aip antibody
    • collaborates/cooperates with ARF (alternate reading frame) protein antibody
    • Collaborator of ARF antibody
    • FLJ20036 antibody
    see all

画像

  • Immunofluorescent staining of Human cell line U-2 OS shows positivity in nucleus and cytoplasm. Recommended concentration of ab122715 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • ab122715, at 1/50 dilution, staining CDKN2AIP in paraffin-embedded Human testis tissue by Immunohistochemistry.
  • All lanes : Anti-CDKN2AIP antibody (ab122715) at 1/250 dilution

    Lane 1 : RT 4 lysate
    Lane 2 : U 251 MG lysate
    Lane 3 : Human plasma lysate
    Lane 4 : Human liver lysate
    Lane 5 : Human tonsil lysate

    Developed using the ECL technique

参考文献

ab122715 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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