製品の概要

  • 製品名Anti-CDKAL1 antibody
    CDKAL1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to CDKAL1
  • アプリケーション適用あり: ICC/IF, WB, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 250 - 350 of Human CDKAL1.

    (Peptide available as ab74019.)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in the following whole cell lysates: HepG2, Jurkat, Raji and SK N BE. This antibody gave a positive result when used in the following formaldehyde fixed cell lines: PANC-1.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーPreservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab74020 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF
WB
IHC-P
  • 追加情報ICC/IF: Use at a concentration of 5 µg/ml.
    WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 65 kDa (predicted molecular weight: 65 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能Catalyzes the methylthiolation of N6-threonylcarbamoyladenosine (t(6)A), leading to the formation of 2-methylthio-N6-threonylcarbamoyladenosine (ms(2)t(6)A) at position 37 in tRNAs that read codons beginning with adenine.
    • 組織特異性Expressed in pancreatic islets.
    • 関連疾患Genetic variations in CDKAL1 are a cause of susceptibility to diabetes mellitus, non-insulin-dependent (NIDDM) [MIM:125853]. A multifactorial disorder of glucose homeostasis caused by a lack of sensitivity to the body's own insulin. Affected individuals usually have an obese body habitus and manifestations of a metabolic syndrome characterized by diabetes, insulin resistance, hypertension and hypertriglyceridemia. The disease results in long-term complications that affect the eyes, kidneys, nerves, and blood vessels.
    • 配列類似性Belongs to the methylthiotransferase family. CDKAL1 subfamily.
      Contains 1 MTTase N-terminal domain.
      Contains 1 TRAM domain.
    • 細胞内局在Endoplasmic reticulum membrane. Is a tail-anchored protein that exploits the TCR40 pathway for insertion into the endoplasmic reticulum.
    • Information by UniProt
    • 参照データベース
    • 別名
      • CDK5 regulatory subunit associated protein 1 like 1 antibody
      • CDK5 regulatory subunit-associated protein 1-like 1 antibody
      • CDKAL_HUMAN antibody
      • CDKAL1 antibody
      • Threonylcarbamoyladenosine tRNA methylthiotransferase antibody
      • tRNA t 6 A37 methylthiotransferase antibody
      • tRNA-t(6)A37 methylthiotransferase antibody
      see all

    Anti-CDKAL1 antibody 画像

    • ICC/IF image of ab74020 stained PANC-1 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab74020 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    • All lanes : Anti-CDKAL1 antibody (ab74020) at 1 µg/ml

      Lane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
      Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
      Lane 3 : Raji (Human Burkitt's lymphoma cell line) Whole Cell Lysate
      Lane 4 : SK N BE (Human neuroblastoma) Whole Cell Lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

      Performed under reducing conditions.

      Predicted band size : 65 kDa
      Observed band size : 65 kDa


      Exposure time : 8 minutes
    • ab74020 staining CDKAL1 in mouse pancreatic tissue sections by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue underwent fixation in formaldehyde, heat mediated antigen retrieval and blocking (5 minutes/peroxidase block and 10 minutes/protein block) for 45 minutes at 200C. The primary antibody was diluted 1/250 and incubated with the sample for 45 minutes at 200C. A HRP conjugated goat polyclonal to rabbit IgG was used undiluted as secondary antibody.

      See Abreview

    • ab74020 staining CDKAL1 in human pancreatic tissue sections by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue underwent fixation in formaldehyde, heat mediated antigen retrieval and blocking (5 minutes/peroxidase block and 10 minutes/protein block) for 15 minutes at 200C. The primary antibody was diluted 1/250 and incubated with the sample for 45 minutes at 200C. A HRP polymer conjugated goat polyclonal to rabbit IgG was used undiluted as secondary antibody.

      See Abreview

    Anti-CDKAL1 antibody (ab74020) 使用論文

    This product has been referenced in:
    • Brambillasca S  et al. CDK5 Regulatory Subunit-associated Protein 1-Like 1 (CDKAL1) Is a Tail-anchored Protein in the Endoplasmic Reticulum (ER) of Insulinoma Cells. J Biol Chem 287:41808-19 (2012). WB . Read more (PubMed: 23048041) »

    See 1 Publication for this product

    Product Wall

    Application Western blot
    Sample Mouse Cell lysate - whole cell (Jurkat, NIH-3T3, GC-1)
    Loading amount 20 µg
    Specification Jurkat, NIH-3T3, GC-1
    Gel Running Conditions Reduced Denaturing
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
    Username

    Mr. Antibody Solutions

    Verified customer

    投稿 May 13 2011

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Human Tissue sections (Pancreas)
    Specification Pancreas
    Fixative Formaldehyde
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Dako Target Retreival Solution
    Permeabilization No
    Blocking step 5 minutes of peroxidase block then 10 minutes of protein block. These are ready-to-use reagents purchased from Dako as blocking agent for 15 minute(s) · Concentration: 100% · Temperature: 20°C
    Username

    Mr. Antibody Solutions

    Verified customer

    投稿 Jan 14 2010

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Mouse Tissue sections (pancreas)
    Specification pancreas
    Fixative Formaldehyde
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Dako Target Retreival Solution
    Permeabilization No
    Blocking step 5 minutes of peroxidase block then 10 minutes of protein block. These are ready-to-use reagents purchased from Dako as blocking agent for 45 minute(s) · Concentration: 100% · Temperature: 20°C
    Username

    Mr. Antibody Solutions

    Verified customer

    投稿 Jan 14 2010

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"