製品の概要

  • 製品名Anti-Cdk9 antibody
    Cdk9 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Cdk9
  • 特異性No reaction is observed against other related cyclin dependent kinases.
  • アプリケーション適用あり: ICC/IF, ELISA, IHC-P, IP, WBmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Multiple synthetic peptides corresponding to C-terminal and N-terminal domains of the protein coded by the human gene cdk9 (PITALRE)

  • 特記事項cdk9(PITALRE) interacts with a conserved domain in the TRAF-C region of the tumor necrosis factor signal transducer TRAF2. The positive transcription elongation factor b (P-TEFb) is identified as cdk9 paired with cyclin T1.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab6544 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use at an assay dependent concentration. PubMed: 21378166
ELISA 1/500 - 1/2000.
IHC-P 1/200 - 1/1000.
IP Use at an assay dependent concentration.
WB 1/500 - 1/1000. Predicted molecular weight: 43 kDa.

ターゲット情報

  • 機能Member of the cyclin-dependent kinase pair (CDK9/cyclin-T) complex, also called positive transcription elongation factor b (P-TEFb), which facilitates the transition from abortive to production elongation by phosphorylating the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNAP II), SUPT5H and RDBP. The CDK9/cyclin-K complex has also a kinase activity toward CTD of RNAP II and can substitute for P-TEFb in vitro.
  • 組織特異性Ubiquitous.
  • 配列類似性Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
    Contains 1 protein kinase domain.
  • 細胞内局在Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • C-2K antibody
    • CDC2 related kinase antibody
    • CDC2L4 antibody
    • Cdk 9 antibody
    • Cdk9 antibody
    • CDK9_HUMAN antibody
    • Cell division cycle 2-like protein kinase 4 antibody
    • Cell division protein kinase 9 antibody
    • CTK1 antibody
    • Cyclin dependent kinase 9 antibody
    • Cyclin-dependent kinase 9 antibody
    • PITALRE antibody
    • Serine/threonine-protein kinase PITALRE antibody
    • TAK antibody
    • Tat associated kinase complex catalytic subunit antibody
    see all

Anti-Cdk9 antibody 画像

  • Immunohistochemical staining of mouse tissue using anti-cdk9 (PITALRE) antiserum. The staining shows the location of cdk9 / PITALRE protein in developing mouse tissue. Arrow indicates area of high expression.

  • Immunofluorescence analysis of HeLa cells, staining Cdk9 (red) with ab6544.

    The cells were fixed with 4% paraformaldehyde and permeabilized with 0.5% Triton X-100 for 10 min at 4°C. The cells were incubated with 0.05% PBS-T before incubating with primary antibody at 1/2000 dilution.

Anti-Cdk9 antibody (ab6544) 使用論文

This product has been referenced in:
  • Zhang H  et al. SIRT2 directs the replication stress response through CDK9 deacetylation. Proc Natl Acad Sci U S A 110:13546-51 (2013). Read more (PubMed: 23898190) »
  • Qi T  et al. G-actin participates in RNA polymerase II-dependent transcription elongation by recruiting positive transcription elongation factor b (P-TEFb). J Biol Chem 286:15171-81 (2011). ICC/IF ; Human . Read more (PubMed: 21378166) »

See all 4 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HeLa Cells)
Loading amount 35 µg
Specification HeLa Cells
Gel Running Conditions Reduced Denaturing (8 % SDS gel)
Blocking step Milk as blocking agent for 45 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Feb 04 2013

Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Xenopus laevis Cell lysate - whole cell (Xenopus laevis gastrula embryo extract (Stage12))
Loading amount 0.5 µg
Specification Xenopus laevis gastrula embryo extract (Stage12)
Gel Running Conditions Reduced Denaturing (10% SDS-PAGE)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 23°C
Username

Dr. Kei Miyamoto

Verified customer

投稿 Oct 10 2012

Thank you very much for your interest in our products.

We have three antibodies which may interest you as they are all tested for both IP and WB.In addition,the homology between the two species is 100% for this protein, therefore,it seems ver...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"