Anti-Cdk9 抗体 (ab6544)
Key features and details
- Rabbit polyclonal to Cdk9
- Suitable for: IHC-P, ELISA, IP, WB
- Reacts with: Mouse, Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-Cdk9 antibody
Cdk9 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Cdk9 -
由来種
Rabbit -
特異性
No reaction is observed against other related cyclin dependent kinases. -
アプリケーション
適用あり: IHC-P, ELISA, IP, WBmore details -
種交差性
交差種: Mouse, Human -
免疫原
Synthetic peptide corresponding to Human Cdk9. Multiple synthetic peptides corresponding to C-terminal and N-terminal domains.
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ポジティブ・コントロール
- WB: human kidney lysate, PC3 lysate IHC: mouse tissue
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特記事項
cdk9(PITALRE) interacts with a conserved domain in the TRAF-C region of the tumor necrosis factor signal transducer TRAF2. The positive transcription elongation factor b (P-TEFb) is identified as cdk9 paired with cyclin T1.The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.01% Sodium azide
Constituents: 0.88% Sodium chloride, 0.42% Potassium phosphate -
Concentration information loading...
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精製度
Whole antiserum -
特記事項(精製)
Sterile filtered. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab6544の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-P |
1/200 - 1/1000.
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ELISA |
Use at an assay dependent concentration.
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IP |
Use at an assay dependent concentration.
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WB | (2) |
Use at an assay dependent concentration. Predicted molecular weight: 43 kDa.
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特記事項 |
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IHC-P
1/200 - 1/1000. |
ELISA
Use at an assay dependent concentration. |
IP
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. Predicted molecular weight: 43 kDa. |
ターゲット情報
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機能
Member of the cyclin-dependent kinase pair (CDK9/cyclin-T) complex, also called positive transcription elongation factor b (P-TEFb), which facilitates the transition from abortive to production elongation by phosphorylating the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNAP II), SUPT5H and RDBP. The CDK9/cyclin-K complex has also a kinase activity toward CTD of RNAP II and can substitute for P-TEFb in vitro. -
組織特異性
Ubiquitous. -
配列類似性
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
Contains 1 protein kinase domain. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 1025 Human
- Entrez Gene: 107951 Mouse
- Omim: 603251 Human
- SwissProt: P50750 Human
- SwissProt: Q99J95 Mouse
- Unigene: 150423 Human
- Unigene: 706809 Human
- Unigene: 27557 Mouse
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別名
- C-2K antibody
- CDC2 related kinase antibody
- CDC2L4 antibody
see all
画像
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All lanes : Anti-Cdk9 antibody (ab6544) at 1/1000 dilution
Lane 1 : Opal PreStain 11-245 kDa (MB-210-0500) at 10 µl
Lane 2 : Human kidney lysate at 5 µg
Lane 3 : PC3 lysate at 20 µg
Lane 4 : Blank well
Predicted band size: 43 kDa
Exposure time: 46 seconds -
Immunohistochemical staining of mouse tissue using anti-cdk9 (PITALRE) antiserum. The staining shows the location of cdk9 / PITALRE protein in developing mouse tissue. Arrow indicates area of high expression.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (8)
ab6544 は 8 報の論文で使用されています。
- Sithole N et al. DDX5 potentiates HIV-1 transcription as a co-factor of Tat. Retrovirology 17:6 (2020). PubMed: 32228614
- Cary DC et al. HIV Transcription is Independent of Mediator Kinases. AIDS Res Hum Retroviruses N/A:N/A (2019). PubMed: 31044597
- Shan J et al. The C/ebp-Atf response element (CARE) location reveals two distinct Atf4-dependent, elongation-mediated mechanisms for transcriptional induction of aminoacyl-tRNA synthetase genes in response to amino acid limitation. Nucleic Acids Res 44:9719-9732 (2016). PubMed: 27471030
- Kuzmina A et al. A single point mutation in cyclin T1 eliminates binding to Hexim1, Cdk9 and RNA but not to AFF4 and enforces repression of HIV transcription. Retrovirology 11:51 (2014). WB . PubMed: 24985467
- Zhang H et al. SIRT2 directs the replication stress response through CDK9 deacetylation. Proc Natl Acad Sci U S A 110:13546-51 (2013). PubMed: 23898190
- Qi T et al. G-actin participates in RNA polymerase II-dependent transcription elongation by recruiting positive transcription elongation factor b (P-TEFb). J Biol Chem 286:15171-81 (2011). ICC/IF ; Human . PubMed: 21378166
- Kruse U et al. Chemoproteomics-based kinome profiling and target deconvolution of clinical multi-kinase inhibitors in primary chronic lymphocytic leukemia cells. Leukemia : (2010). PubMed: 20944678
- Iankova I et al. Peroxisome proliferator-activated receptor gamma recruits the positive transcription elongation factor b complex to activate transcription and promote adipogenesis. Mol Endocrinol 20:1494-505 (2006). PubMed: 16484339