製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to Cdk8
  • 由来種
    Rabbit
  • アプリケーション
    適用あり: IP, IHC-Pmore details
    適用なし: WB
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rabbit, Horse, Chicken, Cow, Dog, Turkey, Xenopus laevis, Chimpanzee, Rhesus monkey, Gorilla, Tilapia, Orangutan, Xenopus tropicalis, Medaka fish
  • 免疫原

    Synthetic peptide, corresponding to a region within amino acids 370-420 of Human Cdk8 (P49336).

  • ポジティブ・コントロール
    • HeLa cell lysates.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab115155 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IP Use at 2-5 µg/mg of lysate.
IHC-P Use at an assay dependent concentration.
  • 追加情報
    Is unsuitable for WB.
  • ターゲット情報

    • 機能
      Component of the Mediator complex, a coactivator involved in regulated gene transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Phosphorylates the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNAp II), which may inhibit the formation of a transcription initiation complex. Phosphorylates CCNH leading to down-regulation of the TFIIH complex and transcriptional repression. Recruited through interaction with MAML1 to hyperphosphorylate the intracellular domain of NOTCH, leading to its degradation.
    • 配列類似性
      Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
      Contains 1 protein kinase domain.
    • 細胞内局在
      Nucleus.
    • Information by UniProt
    • 参照データベース
      see all
    • 別名
      • cdk8 antibody
      • CDK8 protein kinase antibody
      • CDK8_HUMAN antibody
      • Cell Division Kinase 8 antibody
      • Cell division protein kinase 8 antibody
      • Cyclin Dependent kinase 8 antibody
      • Cyclin-dependent kinase 8 antibody
      • K35 antibody
      • Mediator complex subunit cdk8 antibody
      • Mediator of RNA polymerase II transcription subunit cdk8 antibody
      • Protein kinase K35 antibody
      see all

    画像

    • Immunoprecipitation of Cdk8 (3 µg of antibody per mg of HeLa cells lysate; 20% of the IP loaded). Lane 1- Immunoprecipitation with anti-Cdk8 antibody (ab115155); Lane 2-Immunoprecipitation with an anti-Cdk8 antibody that recognizes an upstream epitope; Lane 3- Immunoprecipitation with IgG control. An anti-Cdk8 antibody used in lane 2 was used at 0.4 µg/ml for western blot. Detection: Chemiluminescence with exposure time of 30 sec.

    参考文献

    ab115155 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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