製品の概要

  • 製品名Anti-Cdk8 antibody
    Cdk8 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Cdk8
  • アプリケーション適用あり: WBmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rat, Rabbit, Horse, Dog, Macaque Monkey, Gorilla, Chinese Hamster
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human Cdk8.

  • ポジティブ・コントロール
    • This antibody gave a positive signal in the following whole cell lysates: HeLa; HEK293; HepG2; Caco2; A549; MCF7; NIH3T3.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab106320 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 53 kDa (predicted molecular weight: 53 kDa).

ターゲット情報

  • 機能Component of the Mediator complex, a coactivator involved in regulated gene transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Phosphorylates the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNAp II), which may inhibit the formation of a transcription initiation complex. Phosphorylates CCNH leading to down-regulation of the TFIIH complex and transcriptional repression. Recruited through interaction with MAML1 to hyperphosphorylate the intracellular domain of NOTCH, leading to its degradation.
  • 配列類似性Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
    Contains 1 protein kinase domain.
  • 細胞内局在Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • cdk8 antibody
    • CDK8 protein kinase antibody
    • CDK8_HUMAN antibody
    • Cell Division Kinase 8 antibody
    • Cell division protein kinase 8 antibody
    • Cyclin Dependent kinase 8 antibody
    • Cyclin-dependent kinase 8 antibody
    • K35 antibody
    • Mediator complex subunit cdk8 antibody
    • Mediator of RNA polymerase II transcription subunit cdk8 antibody
    • Protein kinase K35 antibody
    see all

Anti-Cdk8 antibody 画像

  • All lanes : Anti-Cdk8 antibody (ab106320) at 1 µg/ml (Milk Blocked - 5%)

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
    Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 4 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate
    Lane 5 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
    Lane 6 : A549 (Human lung adenocarcinoma epithelial cell line) Whole Cell Lysate
    Lane 7 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 53 kDa
    Observed band size : 53 kDa
    Additional bands at : 78 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 90 seconds

Anti-Cdk8 antibody (ab106320) 使用論文

ab106320 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"