製品の概要

  • 製品名
    Anti-Cdk7 antibody [MO1]
    Cdk7 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [MO1] to Cdk7
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant full length Human Cdk7

  • ポジティブ・コントロール
    • MCF7, HeLa and A431 cell lysates
  • 特記事項

    Abcam is committed to meeting high standards of ethical manufacturing and as such, we will be discontinuing this product, which has been generated by the ascites method, within the next year. We are sorry for any inconvenience this may cause. If you would like help finding an alternative product, please do not hesitate to contact our scientific support team.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab115181 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 39 kDa (predicted molecular weight: 39 kDa). (ECL).

ターゲット情報

  • 機能
    Cyclin-dependent kinases (CDKs) are activated by the binding to a cyclin and mediate the progression through the cell cycle. Each different complex controls a specific transition between two subsequent phases in the cell cycle. CDK7 is the catalytic subunit of the CDK-activating kinase (CAK) complex, a serine-threonine kinase. CAK activates the cyclin-associated kinases CDK1, CDK2, CDK4 and CDK6 by threonine phosphorylation. CAK complexed to the core-TFIIH basal transcription factor activates RNA polymerase II by serine phosphorylation of the repetitive C-terminus domain (CTD) of its large subunit (POLR2A), allowing its escape from the promoter and elongation of the transcripts. Involved in cell cycle control and in RNA transcription by RNA polymerase II. Its expression and activity are constant throughout the cell cycle.
  • 組織特異性
    Ubiquitous.
  • 配列類似性
    Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
    Contains 1 protein kinase domain.
  • 翻訳後修飾
    Phosphorylation of Ser-164 during mitosis inactivates the enzyme.
    Phosphorylation of Thr-170 is required for activity.
  • 細胞内局在
    Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 39 kDa protein kinase antibody
    • CAK antibody
    • CAK1 antibody
    • Cdk activating kinase antibody
    • CDK-activating kinase 1 antibody
    • CDK-activating kinase antibody
    • cdk7 antibody
    • CDK7_HUMAN antibody
    • CDKN7 antibody
    • Cell division protein kinase 7 antibody
    • Cyclin dependent kinase 7 antibody
    • cyclin-dependent kinase 7 (MO15 homolog, Xenopus laevis, cdk-activating kinase) antibody
    • Cyclin-dependent kinase 7 antibody
    • HCAK antibody
    • Homolog of Xenopus MO15 Cdk activating kinase antibody
    • Kinase subunit of CAK antibody
    • MO15 antibody
    • MO15, Xenopus, homolog of antibody
    • P39 Mo15 antibody
    • p39MO15 antibody
    • Serine threonine kinase Stk1 antibody
    • Serine/threonine protein kinase 1 antibody
    • Serine/threonine protein kinase MO15 antibody
    • STK1 antibody
    • TFIIH basal transcription factor complex kinase subunit antibody
    see all

Anti-Cdk7 antibody [MO1] 画像

  • All lanes : Anti-Cdk7 antibody [MO1] (ab115181) at 1 µg/ml

    Lane 1 : MCF7 cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : A431 cell lysate


    Predicted band size : 39 kDa
    Observed band size : 39 kDa

プロトコール

Anti-Cdk7 antibody [MO1] (ab115181) 使用論文

This product has been referenced in:
  • Patel H  et al. Expression of CDK7, Cyclin H, and MAT1 Is Elevated in Breast Cancer and Is Prognostic in Estrogen Receptor-Positive Breast Cancer. Clin Cancer Res 22:5929-5938 (2016). IHC ; Human . Read more (PubMed: 27301701) »

See 1 Publication for this product

Product Wall

There are currently no Abreviews or Questions for ab115181.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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