製品の概要

  • 製品名Anti-Cdk7 antibody [EP4324]
    Cdk7 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EP4324] to Cdk7
  • アプリケーション適用あり: WBmore details
    適用なし: Flow Cyt,ICC/IF,IHC-P or IP
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant fragment corresponding to residues in Human Cdk7.

  • ポジティブ・コントロール
    • MCF7, A431, and HeLa cell lysates.
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

     

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab124712 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/10000. Detects a band of approximately 42 kDa (predicted molecular weight: 39 kDa).
  • 追加情報Is unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.
  • ターゲット情報

    • 機能Cyclin-dependent kinases (CDKs) are activated by the binding to a cyclin and mediate the progression through the cell cycle. Each different complex controls a specific transition between two subsequent phases in the cell cycle. CDK7 is the catalytic subunit of the CDK-activating kinase (CAK) complex, a serine-threonine kinase. CAK activates the cyclin-associated kinases CDK1, CDK2, CDK4 and CDK6 by threonine phosphorylation. CAK complexed to the core-TFIIH basal transcription factor activates RNA polymerase II by serine phosphorylation of the repetitive C-terminus domain (CTD) of its large subunit (POLR2A), allowing its escape from the promoter and elongation of the transcripts. Involved in cell cycle control and in RNA transcription by RNA polymerase II. Its expression and activity are constant throughout the cell cycle.
    • 組織特異性Ubiquitous.
    • 配列類似性Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
      Contains 1 protein kinase domain.
    • 翻訳後修飾Phosphorylation of Ser-164 during mitosis inactivates the enzyme.
      Phosphorylation of Thr-170 is required for activity.
    • 細胞内局在Nucleus.
    • Information by UniProt
    • 参照データベース
    • 別名
      • 39 kDa protein kinase antibody
      • CAK antibody
      • CAK1 antibody
      • Cdk activating kinase antibody
      • CDK-activating kinase 1 antibody
      • CDK-activating kinase antibody
      • cdk7 antibody
      • CDK7_HUMAN antibody
      • CDKN7 antibody
      • Cell division protein kinase 7 antibody
      • Cyclin dependent kinase 7 antibody
      • cyclin-dependent kinase 7 (MO15 homolog, Xenopus laevis, cdk-activating kinase) antibody
      • Cyclin-dependent kinase 7 antibody
      • HCAK antibody
      • Homolog of Xenopus MO15 Cdk activating kinase antibody
      • Kinase subunit of CAK antibody
      • MO15 antibody
      • MO15, Xenopus, homolog of antibody
      • P39 Mo15 antibody
      • p39MO15 antibody
      • Serine threonine kinase Stk1 antibody
      • Serine/threonine protein kinase 1 antibody
      • Serine/threonine protein kinase MO15 antibody
      • STK1 antibody
      • TFIIH basal transcription factor complex kinase subunit antibody
      see all

    Anti-Cdk7 antibody [EP4324] 画像

    • All lanes : Anti-Cdk7 antibody [EP4324] (ab124712) at 1/1000 dilution

      Lane 1 : MCF7 cell lysate
      Lane 2 : A431 cell lysate
      Lane 3 : HeLa cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Lanes 1 - 2 : Goat anti-Rabbit HRP at 1/2000 dilution


      Predicted band size : 39 kDa

    Anti-Cdk7 antibody [EP4324] (ab124712) 使用論文

    ab124712 has not yet been referenced specifically in any publications.

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    There are currently no Abreviews or Questions for ab124712.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"