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RabMAb

Anti-Cdk6 抗体 [EPR4515] (ab124821)

製品の概要

  • 製品名
    Anti-Cdk6 antibody [EPR4515]
    Cdk6 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR4515] to Cdk6
  • アプリケーション
    適用あり: ICC/IF, WB, IHC-P, Flow Cytmore details
    適用なし: IP
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide. within Human Cdk6 aa 300 to the C-terminus (C terminal). The exact sequence is proprietary.
    Database link: Q00534

  • ポジティブ・コントロール
    • WB: Jurkat, K562, HeLa and 293T cell lysates. IHC-P: Human tonsil tissue. ICC/IF: HeLa and wildtype HAP1 cells.
  • 特記事項

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab124821 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF 1/500.
WB 1/50000 - 1/200000. Detects a band of approximately 37 kDa (predicted molecular weight: 37 kDa).
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Flow Cyt 1/100 - 1/1000.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

  • 追加情報
    Is unsuitable for IP.
  • ターゲット情報

    • 機能
      Probably involved in the control of the cell cycle. Interacts with D-type G1 cyclins.
    • 配列類似性
      Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
      Contains 1 protein kinase domain.
    • Information by UniProt
    • 参照データベース
    • 別名
      • CDK 6 antibody
      • CDK6 antibody
      • CDK6_HUMAN antibody
      • Cell division protein kinase 6 antibody
      • Crk 2 antibody
      • Crk2 antibody
      • Cyclin dependent kinase 6 antibody
      • Cyclin-dependent kinase 6 antibody
      • MCPH12 antibody
      • MGC59692 antibody
      • p40 antibody
      • PLSTIRE antibody
      • Serine/threonine protein kinase PLSTIRE antibody
      • Serine/threonine-protein kinase PLSTIRE antibody
      • STQTL11 antibody
      see all

    画像



    • Predicted band size : 37 kDa

      Lanes 1, 3 and 5: Wild-type HAP1 cell lysate (20 µg)
      Lanes 2, 4 and 6: CDK6 knockout HAP1 cell lysate (20 µg)
      Lanes 1 and 2: Green signal from target - ab124821 observed at 37 kDa
      Lanes 3 and 4: Red signal from loading control - ab8226 observed at 42 kDa
      Lanes 5 and 6: Merged (red and green) signal
      ab124821 was shown to specifically react with CDK6 when CDK6 knockout samples were used. Wild-type and CDK6 knockout samples were subjected to SDS-PAGE. ab124821 and ab8226 (loading control to beta actin) were diluted at 1/10 000 and 1/1000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDy 680RD) preadsorbed (ab216776) secondary antibodies at 1/10,000 dilution for 1 h at room temperature before imaging.

    • ab124821 staining Cdk6 in wild-type HAP1 cells (top panel) and Cdk6 knockout HAP1 cells (bottom panel). The cells were fixed with 4% formaldehyde (10min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab124821 at 1/500 dilution and ab195889 at 1/250 dilution (shown in pseudo colour red) overnight at +4°C, followed by a further incubation at room temperature for 1h with a goat secondary antibody to Rabbit IgG (Alexa Fluor® 488) (ab150081) at 2 μg/ml (shown in green). Nuclear DNA was labelled in blue with DAPI.

    • ab124821 at 1/100 dilution, staining Cdk6 in Formalin fixed Paraffin-embedded Human tonsil tissue by Immunohistochemistry.
    • All lanes : Anti-Cdk6 antibody [EPR4515] (ab124821) at 1/1000 dilution

      Lane 1 : Jurkat cell lysate
      Lane 2 : K562 cell lysate
      Lane 3 : HeLa cell lysate
      Lane 4 : 293T cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat-anti-rabbit HRP at 1/2000 dilution

      Predicted band size : 37 kDa
      Observed band size : 37 kDa
    • ICC/IF image of ab124821 at 1/100 dilution, staining Cdk6 in HeLa cells.
    • Overlay histogram showing HeLa cells stained with ab124821 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab124821, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

    参考文献

    This product has been referenced in:
    • Fan Y  et al. CBX3 promotes colon cancer cell proliferation by CDK6 kinase-independent function during cell cycle. Oncotarget 8:19934-19946 (2017). Read more (PubMed: 28193906) »
    • Lv XJ  et al. RNA-binding motif protein 5 inhibits the proliferation of cigarette smoke-transformed BEAS-2B cells through cell cycle arrest and apoptosis. Oncol Rep : (2016). WB ; Mouse . Read more (PubMed: 26782095) »

    See all 10 Publications for this product

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