製品の概要

  • 製品名
  • 製品の詳細
    Mouse monoclonal to Cdk6
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant fragment: KDGLCRADQQ YECVAEIGEG AYGKVFKARD LKNGGRFVAL KRVRVQTGEE GMPLSTIREV AVLRHLETFE HPNVVRLFDV CTVSRTDRET KLTLVFE, corresponding to amino acids 3-100 of Human Cdk6

  • 特記事項

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab54576 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 37 kDa.

ターゲット情報

  • 機能
    Probably involved in the control of the cell cycle. Interacts with D-type G1 cyclins.
  • 配列類似性
    Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
    Contains 1 protein kinase domain.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CDK 6 antibody
    • CDK6 antibody
    • CDK6_HUMAN antibody
    • Cell division protein kinase 6 antibody
    • Crk 2 antibody
    • Crk2 antibody
    • Cyclin dependent kinase 6 antibody
    • Cyclin-dependent kinase 6 antibody
    • MCPH12 antibody
    • MGC59692 antibody
    • p40 antibody
    • PLSTIRE antibody
    • Serine/threonine protein kinase PLSTIRE antibody
    • Serine/threonine-protein kinase PLSTIRE antibody
    • STQTL11 antibody
    see all

画像



  • Predicted band size : 37 kDa

    Lanes 1, 3 and 5: Wild-type HAP1 cell lysate (20 µg)
    Lanes 2, 4 and 6: CDK6 knockout HAP1 cell lysate (20 µg)
    Lanes 1 and 2: Green signal from target – ab54576 observed at 37 kDa
    Lanes 3 and 4: Red signal from loading control – ab8227 observed at 42 kDa
    Lanes 5 and 6: Merged (red and green) signal

    ab54576 was shown to react with CDK6 when CDK6 knockout samples were used, along with additional cross-reactive bands. Wild-type and CDK6 knockout samples were subjected to SDS-PAGE. ab54576 and ab8227 (loading control to beta actin) were diluted 1 µg/mL and 1/2000 respectively and incubated overnight at 4ºC. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) and Goat Anti-Rabbit IgG H&L (IRDye® 680RD)  preadsorbed (ab216777) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.



  • Predicted band size : 37 kDa
    Cdk6 antibody (ab54576) at 1ug/lane + Jurkat cell lysate at 25ug/lane.

プロトコール

参考文献

This product has been referenced in:
  • Laurenti E  et al. CDK6 levels regulate quiescence exit in human hematopoietic stem cells. Cell Stem Cell 16:302-13 (2015). IF . Read more (PubMed: 25704240) »
  • von Witzleben A  et al. Preclinical Characterization of Novel Chordoma Cell Systems and Their Targeting by Pharmocological Inhibitors of the CDK4/6 Cell-Cycle Pathway. Cancer Res 75:3823-31 (2015). Read more (PubMed: 26183925) »

See all 3 Publications for this product

レビューと Q&A

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Although we produce many in-house antibodies, we obtain other antibodies from a wide range of sources. As such the concentration is set by these sources. All stock currently held by us does have a concentration of 0...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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