Rabbit polyclonal to CDK5RAP3
Mouse, Chimpanzee, Rhesus monkey, Chinese hamster, Orangutan, Platypus
Synthetic peptide corresponding to a region between residue 450 and the C-terminus (residue 506) of human CDK5RAP3 (NP_788276.1)
HeLa and 293T whole cell lysates.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.09% Sodium Azide
Constituents: 8mM PBS, 60mM Citrate, 150mM Tris, pH 7-8
Concentration information loading...
Immunogen affinity purified
ab70776 was affinity purified using an epitope specific to CDK5RAP3 immobilized on solid support.
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IP: Use at 2-5µg/mg of lysate.
WB: 1/2000 - 1/10000. Detects a band of approximately 57 kDa (predicted molecular weight: 57 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Potential regulator of CDK5 activity. May be involved in cell proliferation. Regulates CDK5 activity via its interaction with CDK5R1.
Ubiquitous. Expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Isoform 3 is expressed in kidney, liver, skeletal muscle and placenta.
Belongs to the CDK5RAP3 family.
Phosphorylated in vitro by CDK5.
Information by UniProt
CDK5 activator binding protein C53 antibody
CDK5 activator-binding protein C53 antibody
Western blot - Anti-CDK5RAP3 antibody (ab70776)
All lanes : Anti-CDK5RAP3 antibody (ab70776) at 0.1 µg/ml Lane 1 : HeLa whole cell lysate at 50 µg Lane 2 : HeLa whole cell lysate at 15 µg Lane 3 : HeLa whole cell lysate at 5 µg Lane 4 : 293T whole cell lysate at 50 µg Predicted band size: 57 kDa Observed band size: 57 kDa Additional bands at: 220 kDa, 28 kDa. We are unsure as to the identity of these extra bands. Exposure time: 3 minutes
Immunoprecipitation - Anti-CDK5RAP3 antibody (ab70776)
Detection of CDK5RAP3 by Western Blot of Immunprecipitate.
ab70776 at 1µg/ml staining CDK5RAP3 in HeLa whole cell lysate immunoprecipitated using ab70776 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Chemiluminescence with exposure time of 30 seconds.
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