製品の概要

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab40773 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-Fr 1/200.
WB 1/2000 - 1/10000. Detects a band of approximately 33 kDa (predicted molecular weight: 33 kDa).
IHC-P Use at an assay dependent concentration.
ICC 1/100 - 1/250.
Flow Cyt 1/30.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IP 1/50.

ターゲット情報

  • 機能Probably involved in the control of the cell cycle. Interacts with D1 and D3-type G1 cyclins. Can phosphorylate histone H1, tau, MAP2 and NF-H and NF-M. Also interacts with p35 which activates the kinase.
  • 配列類似性Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
    Contains 1 protein kinase domain.
  • 細胞内局在Cytoplasm. Cell projection > lamellipodium. Cell projection > growth cone. In axonal growth cone with extension to the peripheral lamellipodia.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Cdk 5 antibody
    • Cdk5 antibody
    • CDK5_HUMAN antibody
    • Cell division protein kinase 5 antibody
    • Crk6 antibody
    • Cyclin dependent kinase 5 antibody
    • Cyclin-dependent kinase 5 antibody
    • Protein kinase CDK5 splicing antibody
    • PSSALRE antibody
    • Serine threonine protein kinase PSSALRE antibody
    • Serine/threonine-protein kinase PSSALRE antibody
    • Tau protein kinase II catalytic subunit antibody
    • TPKII catalytic subunit antibody
    see all

Anti-Cdk5 antibody [EP715Y] 画像

  • Immunohistochemical analysis of frozen PFA-fixed chicken midbrain tissue cyrosection, labelling Cdk5 with ab40773 at a dilution of 1/200 incubated for 16 hours at 4°C in 0.1% PBS Triton X and 3% BSA. Blocking was with 3% BSA incubated for 1 hour at 21°C. Secondary was a goat anti-rabbit polyclonal Alexa Fluor® 488 conjugate at 1/1000.

  • Anti-Cdk5 antibody [EP715Y] (ab40773) at 1/10000 dilution + HeLa cell lysate

    Predicted band size : 33 kDa
    Observed band size : 33 kDa
  • Paraffin-embedded human brain stained for Cdk5 with ab40773 at 1/100 dilution
  • Flow cytometry analysis of HeLa cells labelling Cdk5 with ab40773 (red). Green - negative control Rabbit IgG.

  • ab40773 at 1/50 immunoprecipitating Cdk5 in HeLa whole cell lysate.

    For western blotting, the primary antibody was used at a dilution of 1/1000 and a HRP-conjugated goat anti-rabbit IgG was used as the secondary antibody (1/1000).

    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

Anti-Cdk5 antibody [EP715Y] (ab40773) 使用論文

This product has been referenced in:
  • Yu HP  et al. TIGAR regulates DNA damage and repair through pentosephosphate pathway and Cdk5-ATM pathway. Sci Rep 5:9853 (2015). WB . Read more (PubMed: 25928429) »
  • Zhang HH  et al. Increased synaptophysin is involved in inflammation-induced heat hyperalgesia mediated by cyclin-dependent kinase 5 in rats. PLoS One 7:e46666 (2012). IHC-FoFr ; Rat . Read more (PubMed: 23056393) »

See all 6 Publications for this product

Product Wall

Application Immunohistochemistry (Frozen sections)
Sample Chicken Tissue (Midbrain cryosection)
Permeabilization Yes - 0.1% PBS Triton X
Specification Midbrain cryosection
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
Fixative Paraformaldehyde
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投稿 Nov 12 2015

Application Western blot
Loading amount 10 µg
Gel Running Conditions Reduced Denaturing (10% SDS gel)
Sample Human Cell lysate - whole cell (human iPS cell derived neurons (healthy/diseased))
Specification human iPS cell derived neurons (healthy/diseased)
Treatment quinolinic acid 72h (and non treated) 500nM
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
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投稿 Jun 17 2014

Application Western blot
Loading amount 40 µg
Gel Running Conditions Reduced Denaturing (10%)
Sample Human Cell lysate - whole cell (HeLa cell line)
Specification HeLa cell line
Blocking step BSA as blocking agent for 45 minute(s) · Concentration: 5% · Temperature: 23°C
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投稿 Dec 12 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing
Sample Human Cell lysate - whole cell (U2OS cells)
Specification U2OS cells
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
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投稿 Jul 12 2013

DISCOUNT CODE: *** Expiration date: April 1st, 2012 I am very pleased to hear you would like to accept our offer and test ab40773 in IHC-FoFr. This code will give you 1 free primary antibody before the expiration date. To redeem this offer, please subm...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HEK 293)
Loading amount 20 µg
Specification HEK 293
Gel Running Conditions Non-reduced Denaturing (12.5)
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
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投稿 Aug 22 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"