製品の概要

  • 製品名Anti-Cdk2 (phospho T39) antibody
    Cdk2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Cdk2 (phospho T39)
  • アプリケーション適用あり: ICC/IF, WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Sheep, Rabbit, Goat, Chicken, Hamster, Cow, Zebrafish, Goldfish
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human Cdk2.

  • ポジティブ・コントロール
    • This antibody gave a positive signal in the following whole cell lysates: A431; Jurkat; HepG2.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab79379 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 10 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 34 kDa).

ターゲット情報

  • 機能Involved in the control of the cell cycle. Interacts with cyclins A, B1, B3, D, or E. Activity of CDK2 is maximal during S phase and G2.
  • 配列類似性Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
    Contains 1 protein kinase domain.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Cdc2 related protein kinase antibody
    • cdc2-related protein kinase antibody
    • CDC28 antibody
    • CDC2A antibody
    • Cdk 2 antibody
    • CDK1 antibody
    • CDK2 antibody
    • CDK2_HUMAN antibody
    • CDKN2 antibody
    • Cell devision kinase 2 antibody
    • Cell division protein kinase 2 antibody
    • Cyclin dependent kinase 2 antibody
    • cyclin dependent kinase 2-alpha antibody
    • Cyclin-dependent kinase 2 antibody
    • kinase Cdc2 antibody
    • MPF antibody
    • p33 protein kinase antibody
    • p33(CDK2) antibody
    see all

Anti-Cdk2 (phospho T39) antibody 画像

  • All lanes : Anti-Cdk2 (phospho T39) antibody (ab79379) at 1 µg/ml

    Lane 1 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : A431 (Human epithelial carcinoma cell lines) EGF Stimulated Whole Cell Lysate
    Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 4 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 5 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate with Immunising peptide at 1 µg/ml
    Lane 6 : A431 (Human epithelial carcinoma cell lines) EGF Stimulated Whole Cell Lysate with Immunising peptide at 1 µg/ml
    Lane 7 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysat with Immunising peptide at 1 µg/ml
    Lane 8 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate with Immunising peptide at 1 µg/ml
    Lane 9 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate with Non-Modified Control Peptide at 1 µg/ml
    Lane 10 : A431 (Human epithelial carcinoma cell lines) EGF Stimulated Whole Cell Lysate with Non-Modified Control Peptide at 1 µg/ml
    Lane 11 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate with Non-Modified Control Peptide at 1 µg/ml
    Lane 12 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate with Non-Modified Control Peptide at 1 µg/ml

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 34 kDa
    Observed band size : 37 kDa (why is the actual band size different from the predicted?)


    Exposure time : 4 minutes
  • ICC/IF image of ab79379 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab79379 at 10µg/ml overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti- rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in Methanol (100%, 5 minutes) fixed HepG2 cells at 10ug/ml

Anti-Cdk2 (phospho T39) antibody (ab79379) 使用論文

ab79379 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab79379.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"