製品の概要

  • 製品名Anti-Cdk2 (phospho T14) antibody [EP2234Y]
    Cdk2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EP2234Y] to Cdk2 (phospho T14)
  • 特異性This antibody detects Cdk2 phosphorylated on Threonine 14.
  • アプリケーション適用あり: WB, IP, IHC-P, ICCmore details
    適用なし: Flow Cyt
  • 種交差性
    交差種: Rat, Human, Chinese Hamster
  • 免疫原

    A synthetic phospho-peptide corresponding to residues surrounding Thr14 of human Cdk2.

  • ポジティブ・コントロール
    • HeLa cell lysate, human tonsil tissue. IHC/P: Rat esophagus (FFPE)
  • 特記事項

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    A trial size is available to purchase for this antibody.

    Mouse: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab68265 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/10000. Detects a band of approximately 34 kDa (predicted molecular weight: 34 kDa).
IP 1/30.
IHC-P 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC 1/100 - 1/250.
  • 追加情報Is unsuitable for Flow Cyt.
  • ターゲット情報

    • 機能Involved in the control of the cell cycle. Interacts with cyclins A, B1, B3, D, or E. Activity of CDK2 is maximal during S phase and G2.
    • 配列類似性Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
      Contains 1 protein kinase domain.
    • Information by UniProt
    • 参照データベース
    • 別名
      • Cdc2 related protein kinase antibody
      • cdc2-related protein kinase antibody
      • CDC28 antibody
      • CDC2A antibody
      • Cdk 2 antibody
      • CDK1 antibody
      • CDK2 antibody
      • CDK2_HUMAN antibody
      • CDKN2 antibody
      • Cell devision kinase 2 antibody
      • Cell division protein kinase 2 antibody
      • Cyclin dependent kinase 2 antibody
      • cyclin dependent kinase 2-alpha antibody
      • Cyclin-dependent kinase 2 antibody
      • kinase Cdc2 antibody
      • MPF antibody
      • p33 protein kinase antibody
      • p33(CDK2) antibody
      see all

    Anti-Cdk2 (phospho T14) antibody [EP2234Y] 画像

    • IHC image of ab68265 staining Cdk2 in rat esophagus formalin fixed paraffin embedded tissue sections, performed on a Leica Bond. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab68265, 1:250 dilution, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. No primary antibody was used in the secondary only control (shown on the inset).
      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    • All lanes : Anti-Cdk2 (phospho T14) antibody [EP2234Y] (ab68265) at 1/20000 dilution

      Lane 1 : HeLa cell lysate, untreated.
      Lane 2 : HeLa cell lysate, treated with hydroxyurea.

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP conjugated Goat anti-rabbit at 1/2000 dilution

      Predicted band size : 34 kDa
      Observed band size : ~34 kDa (why is the actual band size different from the predicted?)
    • Human tonsil tissue stained with ab68265 at 1/100-1/250 dilution.

    Anti-Cdk2 (phospho T14) antibody [EP2234Y] (ab68265) 使用論文

    This product has been referenced in:
    • Li J  et al. Phosphorylation of MCM3 Protein by Cyclin E/Cyclin-dependent Kinase 2 (Cdk2) Regulates Its Function in Cell Cycle. J Biol Chem 286:39776-85 (2011). WB ; Human . Read more (PubMed: 21965652) »
    • Abdullah C  et al. Expression analysis and molecular targeting of cyclin-dependent kinases in advanced melanoma. Cell Cycle 10:977-88 (2011). WB ; Human . Read more (PubMed: 21358262) »

    See all 3 Publications for this product

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    There are currently no Abreviews or Questions for ab68265.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"