製品の概要

  • 製品名Anti-Cdk2 antibody [1C4]
    Cdk2 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [1C4] to Cdk2
  • アプリケーション適用あり: WB, Flow Cyt, ICC/IFmore details
  • 種交差性
    交差種: Human, Monkey
  • 免疫原

    Recombinant full length Human Cdk2 protein produced in HEK293T cells (NP_001789).

  • ポジティブ・コントロール
    • WB: Cdk2-transfected HEK293T cell lysate, HeLa, SVT2, COS7 and Jurkat cell extracts ICC/IF: HeLa cells and COS7 cells transiently transfected by Cdk2 Flow Cyt: Cdk2-transfected HEK293T cells
  • 特記事項Dilute in PBS (pH 7.3) before use. Stable for 12 months from date of receipt.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab124338 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/2000. Predicted molecular weight: 34 kDa.
Flow Cyt 1/100. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
ICC/IF 1/100.

ターゲット情報

  • 機能Involved in the control of the cell cycle. Interacts with cyclins A, B1, B3, D, or E. Activity of CDK2 is maximal during S phase and G2.
  • 配列類似性Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
    Contains 1 protein kinase domain.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Cdc2 related protein kinase antibody
    • cdc2-related protein kinase antibody
    • CDC28 antibody
    • CDC2A antibody
    • Cdk 2 antibody
    • CDK1 antibody
    • CDK2 antibody
    • CDK2_HUMAN antibody
    • CDKN2 antibody
    • Cell devision kinase 2 antibody
    • Cell division protein kinase 2 antibody
    • Cyclin dependent kinase 2 antibody
    • cyclin dependent kinase 2-alpha antibody
    • Cyclin-dependent kinase 2 antibody
    • kinase Cdc2 antibody
    • MPF antibody
    • p33 protein kinase antibody
    • p33(CDK2) antibody
    see all

Anti-Cdk2 antibody [1C4] 画像

  • All lanes : Anti-Cdk2 antibody [1C4] (ab124338) at 1/500 dilution

    Lane 1 : HEK293T cell lysate transfected with pCMV6-ENTRY control
    Lane 2 : HEK293T cell lysate transfected with pCMV6-ENTRY Cdk2 cDNA

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 34 kDa
    HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC200494)
  • All lanes : Anti-Cdk2 antibody [1C4] (ab124338) at 1/500 dilution

    Lane 1 : HepG2 cell extract
    Lane 2 : HeLa cell extract
    Lane 3 : SVT2 cell extract
    Lane 4 : A549 cell extract
    Lane 5 : COS7 cell extract
    Lane 6 : Jurkat cell extract
    Lane 7 : MDCK cell extract
    Lane 8 : PC12 cell extract
    Lane 9 : MCF7 cell extract

    Lysates/proteins at 35 µg per lane.


    Predicted band size : 34 kDa
    HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC200494)
  • ab124338, at a 1/100 dilution, staining Cdk2 in COS7 cells transiently transfected using pCMV6-ENTRY Cdk2, by Immunofluorescence.
  • ab124338, at a 1/100 dilution, staining Cdk2 in HeLa cells by Immunofluorescence.
  • ab124338, at a 1/100 dilution, staining Cdk2 in HEK293T cells transfected with either pCMV6-ENTRY Cdk2 overexpress plasmid (red) or empty vector control plasmid (blue), by Flow Cytometry.

Anti-Cdk2 antibody [1C4] (ab124338) 使用論文

ab124338 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"