Anti-CDK1 (phospho Y15) 抗体 (ab47594)
製品の概要
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製品名Anti-CDK1 (phospho Y15) antibody
CDK1 一次抗体 製品一覧 -
製品の詳細Rabbit polyclonal to CDK1 (phospho Y15)
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特異性This antibody is specific for CDK1.
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アプリケーション適用あり: WB, ELISAmore details
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種交差性交差種: Mouse, Rat, Human
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免疫原
Synthetic peptide corresponding to Human CDK1 aa 5-54.
Database link: P06493 -
ポジティブ･コントロール
- HepG2 cell extract.
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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バッファーPreservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4 -
Concentration information loading... -
精製度Immunogen affinity purified
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特記事項（精製）The antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site.
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ポリ/モノポリクローナル
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アイソタイプIgG
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研究分野
関連製品
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Compatible Secondaries
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Positive Controls
アプリケーション
Our Abpromise guarantee covers the use of ab47594 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | 1/500 - 1/1000. Detects a band of approximately 35 kDa (predicted molecular weight: 34 kDa). | |
| ELISA | 1/10000. |
ターゲット情報
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機能Plays a key role in the control of the eukaryotic cell cycle. It is required in higher cells for entry into S-phase and mitosis. p34 is a component of the kinase complex that phosphorylates the repetitive C-terminus of RNA polymerase II.
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組織特異性Isoform 2 is found in breast cancer tissues.
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配列類似性Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
Contains 1 protein kinase domain. -
細胞内局在Nucleus.
- Information by UniProt
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参照データベース
- Entrez Gene: 983 Human
- Entrez Gene: 12534 Mouse
- Entrez Gene: 54237 Rat
- Omim: 116940 Human
- SwissProt: P06493 Human
- SwissProt: P11440 Mouse
- SwissProt: P39951 Rat
- Unigene: 334562 Human
see all -
製品の状態CDK1 can be located to the Nucleus, cytoplasm and Mithocondria. It's cytoplasmic during interphase and reversibly translocated from cytoplasm to the nucleus when phosphorilated before G2-M transition when associated with cyclin-B1. Accumulates in mitochondria in G2-arrested cells upon DNA-damage.
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別名
- Cdc 2 antibody
- Cdc2 antibody
- CDC28A antibody
see all
Anti-CDK1 (phospho Y15) antibody 画像
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Western blot - Anti-CDK1 (phospho Y15) antibody (ab47594)Anti-CDK1 (phospho Y15) antibody (ab47594) at 1/500 dilution + COS7 cells
Predicted band size : 34 kDa -
Western blot - Anti-CDK1 (phospho Y15) antibody (ab47594)Anti-CDK1 (phospho Y15) antibody (ab47594) at 1/500 dilution + HepG2 cell extract
Predicted band size : 34 kDa
Observed band size : 35 kDa (why is the actual band size different from the predicted?) -
Western blot - Anti-CDK1 (phospho Y15) antibody (ab47594)This image is courtesy of an anonymous abreview.All lanes : Anti-CDK1 (phospho Y15) antibody (ab47594) at 1/1000 dilution
Lane 1 : untreated HeLa cell lysate
Lane 2 : HeLa cell lysate treated with nocodazole (500ng/mL) for 16 h
Lysates/proteins at 20 µg per lane.
Secondary
polyclonal goat HRP conjugated at 1/2000 dilution
Developed using the ECL technique
Predicted band size : 34 kDa
Observed band size : 34 kDa
Exposure time : 1 minuteThis image is courtesy of an anonymous abreview.
Blocking: 5% milk for 1 hour at 25°C.
プロトコール
Anti-CDK1 (phospho Y15) antibody (ab47594) 使用論文
This product has been referenced in:
- Chang L et al. Targeting Rad50 sensitizes human nasopharyngeal carcinoma cells to radiotherapy. BMC Cancer 16:190 (2016). WB ; Human . Read more (PubMed: 26951044) »
- Li HB et al. Mitochondrial Ribosomal Protein L10 Associates with Cyclin B1/Cdk1 Activity and Mitochondrial Function. DNA Cell Biol 35:680-690 (2016). WB ; Human . Read more (PubMed: 27726420) »
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"