製品の概要

  • 製品名
    Anti-CDK1 (phospho Y15) antibody
    CDK1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to CDK1 (phospho Y15)
  • 由来種
    Rabbit
  • 特異性
    This antibody is specific for CDK1.
  • アプリケーション
    適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide corresponding to Human CDK1 aa 5-54.
    Database link: P06493

  • ポジティブ･コントロール
    • HepG2 cell extract.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab47594 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/1000. Detects a band of approximately 35 kDa (predicted molecular weight: 34 kDa).
ELISA 1/10000.

ターゲット情報

  • 機能
    Plays a key role in the control of the eukaryotic cell cycle. It is required in higher cells for entry into S-phase and mitosis. p34 is a component of the kinase complex that phosphorylates the repetitive C-terminus of RNA polymerase II.
  • 組織特異性
    Isoform 2 is found in breast cancer tissues.
  • 配列類似性
    Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
    Contains 1 protein kinase domain.
  • 細胞内局在
    Nucleus.
  • Information by UniProt
  • 参照データベース
    see all
  • 製品の状態
    CDK1 can be located to the Nucleus, cytoplasm and Mithocondria. It's cytoplasmic during interphase and reversibly translocated from cytoplasm to the nucleus when phosphorilated before G2-M transition when associated with cyclin-B1. Accumulates in mitochondria in G2-arrested cells upon DNA-damage.
  • 別名
    • Cdc 2 antibody
    • Cdc2 antibody
    • CDC28A antibody
    • CDK 1 antibody
    • CDK1 antibody
    • CDK1_HUMAN antibody
    • CDKN1 antibody
    • CELL CYCLE CONTROLLER CDC2 antibody
    • Cell division control protein 2 antibody
    • Cell division control protein 2 homolog antibody
    • Cell division cycle 2 G1 to S and G2 to M antibody
    • Cell division protein kinase 1 antibody
    • Cell Divsion Cycle 2 Protein antibody
    • Cyclin Dependent Kinase 1 antibody
    • Cyclin-dependent kinase 1 antibody
    • DKFZp686L20222 antibody
    • MGC111195 antibody
    • p34 Cdk1 antibody
    • p34 protein kinase antibody
    • P34CDC2 antibody
    see all

画像

  • Western blot - Anti-CDK1 (phospho Y15) antibody (ab47594)
    Western blot - Anti-CDK1 (phospho Y15) antibody (ab47594)
    Anti-CDK1 (phospho Y15) antibody (ab47594) at 1/500 dilution + COS7 cells

    Predicted band size: 34 kDa

  • Western blot - Anti-CDK1 (phospho Y15) antibody (ab47594)
    Western blot - Anti-CDK1 (phospho Y15) antibody (ab47594)
    Anti-CDK1 (phospho Y15) antibody (ab47594) at 1/500 dilution + HepG2 cell extract

    Predicted band size: 34 kDa
    Observed band size: 35 kDa (why is the actual band size different from the predicted?)

  • Western blot - Anti-CDK1 (phospho Y15) antibody (ab47594)
    Western blot - Anti-CDK1 (phospho Y15) antibody (ab47594)This image is courtesy of an anonymous abreview.
    All lanes : Anti-CDK1 (phospho Y15) antibody (ab47594) at 1/1000 dilution

    Lane 1 : untreated HeLa cell lysate
    Lane 2 : HeLa cell lysate treated with nocodazole (500ng/mL) for 16 h

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : polyclonal goat HRP conjugated at 1/2000 dilution

    Developed using the ECL technique.

    Predicted band size: 34 kDa
    Observed band size: 34 kDa


    Exposure time: 1 minute


    Blocking: 5% milk for 1 hour at 25°C.

    See Abreview

プロトコール

参考文献

This product has been referenced in:
  • Chang L  et al. Targeting Rad50 sensitizes human nasopharyngeal carcinoma cells to radiotherapy. BMC Cancer 16:190 (2016). WB ; Human . Read more (PubMed: 26951044) »
  • Li HB  et al. Mitochondrial Ribosomal Protein L10 Associates with Cyclin B1/Cdk1 Activity and Mitochondrial Function. DNA Cell Biol 35:680-690 (2016). WB ; Human . Read more (PubMed: 27726420) »

See all 16 Publications for this product

ab47594 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

レビューと Q&A

レビューを送る 質問を送る

2 reviews or Q&A

10
Votes: Highest First

Western blot abreview for Anti-CDK1 (phospho Y15) antibody

 Excellent
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa cells)
Loading amount
20 µg
Specification
HeLa cells
Treatment
1 - untreated, 2- nocodazole 500 ng/ml 16 h
Gel Running Conditions
Reduced Denaturing (8% Tris Glycine)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More
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Verified customer

投稿 Oct 13 2010

Immunocytochemistry/ Immunofluorescence abreview for Anti-CDK1 (phospho Y15) antibody

 Poor
Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa cells)
Specification
HeLa cells
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.05% Triton X 100 in PBS for 5 min
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More
abreview image
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Abcam user community

Verified customer

投稿 Oct 06 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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