Anti-CDK1 (phospho T14) 抗体 (ab58509)
Key features and details
- Rabbit polyclonal to CDK1 (phospho T14)
- Suitable for: WB, ICC/IF
- Reacts with: Human, African green monkey
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-CDK1 (phospho T14) antibody
CDK1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to CDK1 (phospho T14) -
由来種
Rabbit -
特異性
ab58509 peptide sequence is 100% identical to the sequence of CDK1 and CDK2. Therefore, this antibody is predicted to recognize both proteins.
Detects endogenous levels of CDC2 only when phosphorylated at threonine 14.
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アプリケーション
適用あり: WB, ICC/IFmore details -
種交差性
交差種: Human, African green monkey
交差が予測される動物種: Mouse, Rat -
免疫原
Synthetic peptide corresponding to Human CDK1 aa 1-100 (phospho T14).
Database link: P06493 -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Stable for 12 months at -20°C. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab58509の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/500 - 1/1000. Detects a band of approximately 32 kDa (predicted molecular weight: 34 kDa).
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ICC/IF |
1/100 - 1/500.
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特記事項 |
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WB
1/500 - 1/1000. Detects a band of approximately 32 kDa (predicted molecular weight: 34 kDa). |
ICC/IF
1/100 - 1/500. |
ターゲット情報
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機能
Plays a key role in the control of the eukaryotic cell cycle. It is required in higher cells for entry into S-phase and mitosis. p34 is a component of the kinase complex that phosphorylates the repetitive C-terminus of RNA polymerase II. -
組織特異性
Isoform 2 is found in breast cancer tissues. -
配列類似性
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
Contains 1 protein kinase domain. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 983 Human
- Entrez Gene: 12534 Mouse
- Entrez Gene: 54237 Rat
- Omim: 116940 Human
- SwissProt: P06493 Human
- SwissProt: P11440 Mouse
- SwissProt: P39951 Rat
- Unigene: 334562 Human
see all -
製品の状態
CDK1 can be located to the Nucleus, cytoplasm and Mithocondria. It's cytoplasmic during interphase and reversibly translocated from cytoplasm to the nucleus when phosphorilated before G2-M transition when associated with cyclin-B1. Accumulates in mitochondria in G2-arrested cells upon DNA-damage. -
別名
- Cdc 2 antibody
- Cdc2 antibody
- CDC28A antibody
see all
画像
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Western blot analysis of lysates from HepG2 cells treated with Forskolin 40nM for 30 mins, using CDK1 (phospho T14) Antibody (ab58509). The lane on the right is blocked with the phospho peptide.
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Immunofluorescence analysis of COS7 cells using ab58509 at 1/1000 dilution.
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All lanes : Anti-CDK1 (phospho T14) antibody (ab58509) at 1/500 dilution
Lane 1 : Extracts from HepG2 cells,
treated with Forskolin (40nM, 30mins)
Lane 2 : Extracts from HepG2 cells,
treated with Forskolin (40nM, 30mins), plus immunising peptide
Predicted band size: 34 kDa
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (3)
ab58509 は 3 報の論文で使用されています。
- Gallo D et al. CCNE1 amplification is synthetic lethal with PKMYT1 kinase inhibition. Nature 604:749-756 (2022). PubMed: 35444283
- Jin H et al. A distinct class of plant and animal viral proteins that disrupt mitosis by directly interrupting the mitotic entry switch Wee1-Cdc25-Cdk1. Sci Adv 6:eaba3418 (2020). PubMed: 32426509
- Chow JP & Poon RY The CDK1 inhibitory kinase MYT1 in DNA damage checkpoint recovery. Oncogene : (2012). PubMed: 23146904