製品の概要

  • 製品名Anti-CDK1 antibody [A17]
    CDK1 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [A17] to CDK1
  • 特異性The antibody inhibits the activation of p34cdc2 kinase by cyclins. A study published in the Biotechniques journal suggests that ab18 cross-reacts with Cep152 protein. This should not be a problem for simple western blot experiments because of the difference in molecular weight (MW) between Cdk1 and Cep152. (Cdk1 has a predicted MW of 34-kDa. Isoforms 1-4 of Cep152 have predicted MW of 147, 158, 189 and 196-kDa respectively.) However, this could potentially cause misleading results in Immunofluorescence and pull down experiments. Abcam are currently investigating this further and welcome customer feedback.
  • アプリケーション適用あり: ICC/IF, Flow Cyt, WB, IP, ELISA, IHC-FoFr, IHC-P, RIA, IHC-Frmore details
  • 種交差性
    交差種: Mouse, Rat, Chicken, Human, Xenopus laevis
  • 免疫原

    Recombinant fragment corresponding to Xenopus laevis CDK1 aa 50 to the C-terminus (C terminal).
    Database link: P24033

  • エピトープThe epitope is thought to be residues 220-227 of mouse cdc2, LGTPNNEV.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab18 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use at an assay dependent concentration.
Flow Cyt Use 0.5µg for 106 cells.
WB Use at an assay dependent concentration. Predicted molecular weight: 34 kDa.
IP Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.
IHC-FoFr Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.
RIA Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.

ターゲット情報

  • 機能Plays a key role in the control of the eukaryotic cell cycle. It is required in higher cells for entry into S-phase and mitosis. p34 is a component of the kinase complex that phosphorylates the repetitive C-terminus of RNA polymerase II.
  • 組織特異性Isoform 2 is found in breast cancer tissues.
  • 配列類似性Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
    Contains 1 protein kinase domain.
  • 細胞内局在Nucleus.
  • Information by UniProt
  • 参照データベース
  • 製品の状態CDK1 can be located to the Nucleus, cytoplasm and Mithocondria. It's cytoplasmic during interphase and reversibly translocated from cytoplasm to the nucleus when phosphorilated before G2-M transition when associated with cyclin-B1. Accumulates in mitochondria in G2-arrested cells upon DNA-damage.
  • 別名
    • Cdc 2 antibody
    • Cdc2 antibody
    • CDC28A antibody
    • CDK 1 antibody
    • CDK1 antibody
    • CDK1_HUMAN antibody
    • CDKN1 antibody
    • CELL CYCLE CONTROLLER CDC2 antibody
    • Cell division control protein 2 antibody
    • Cell division control protein 2 homolog antibody
    • Cell division cycle 2 G1 to S and G2 to M antibody
    • Cell division protein kinase 1 antibody
    • Cell Divsion Cycle 2 Protein antibody
    • Cyclin Dependent Kinase 1 antibody
    • Cyclin-dependent kinase 1 antibody
    • DKFZp686L20222 antibody
    • MGC111195 antibody
    • p34 Cdk1 antibody
    • p34 protein kinase antibody
    • P34CDC2 antibody
    see all

Anti-CDK1 antibody [A17] 画像

  • All lanes : Anti-CDK1 antibody [A17] (ab18) at 5 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 3 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
    Lane 4 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 5 : RAW 264.7 (Mouse leukaemic monocyte macrophage cell line) Whole Cell Lysate
    Lane 6 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Rabbit Anti-Mouse IgG H&L (HRP) (ab97046) at 1/10000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 34 kDa
    Observed band size : 35 kDa (why is the actual band size different from the predicted?)


    Exposure time : 4 minutes

    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab18 overnight at 4°C. Antibody binding was detected using an anti-mouse antibody conjugated to HRP, and visualised using ECL development solution.

  • Ab18 staining human skin. Staining is localized to the cytoplasm and nucleus.
    Left panel: with primary antibody at 4 ug/ml. Right panel: isotype control.
    Sections were stained using an automated system (Dako PT Link), at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 antigen retrieval buffer, EDTA pH 9.0. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
  • Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling CDK1 (green) with ab18 at 1/500. Cells were fixed with paraformaldehyde and permeabilized with 0.05% Triton X-100 in PBS for 5 minutes. Blocked with 5% goat serum as blocking agent for 1 hour at 25°C. Alexa Fluor® 488-conjugated goat anti-mouse IgG was used as the secondary antibody. DAPI (blue) was used as a nuclear counterstain.

     

     

    See Abreview

  • Overlay histogram showing MCF7 cells stained with ab18 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab18, 0.5µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in MCF7 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

  • Immunohistochemical analysis of Human lung cancer tissue, staining CDK1 with ab18 at 1/100 dilution.

Anti-CDK1 antibody [A17] (ab18) 使用論文

This product has been referenced in:
  • Munday DC  et al. Interactome analysis of the human respiratory syncytial virus RNA polymerase complex identifies protein chaperones as important cofactors that promote L-protein stability and RNA synthesis. J Virol 89:917-30 (2015). WB ; Human . Read more (PubMed: 25355874) »
  • Cao L  et al. Cyclin-dependent kinase 5 decreases in gastric cancer and its nuclear accumulation suppresses gastric tumorigenesis. Clin Cancer Res 21:1419-28 (2015). Read more (PubMed: 25609066) »

See all 22 Publications for this product

Product Wall

Der Antikörper sollte dies eigentlich unbeschadet überstanden haben. Wir versenden unsere Antikörper so z.B. auch nach Australien oder Thailand, und da kann es schon einmal zu Transitzeiten von einer Woche kommen, und bisher haben wir noch keine erhöht...

Read More

Vielen Dank für IhreAnfrage und all dieInformationen.

Es tut mir leid zu hören, dass dieses Röhrchen vonab18 keine guten Ergebnisse liefert. Ich stimme Ihnen zu, dass kein spezifisches Signal erkannt werden kann in all den von...

Read More

Thank you for your email. I have just been informed by my colleague that the blocking peptide is out of stock. Therefore they will not be able to publish the datasheet hence we are unable to fulfil your request this time. I have done my best to publish...

Read More

Thank you for your email. I have sent request to datasheet team for publishing the datasheet of blocking peptide. They will be able to publish this on Monday next week. I will then send you the catalogue number for ordering. Many thanks for having pati...

Read More

Thank you for contacting us. Isotype controls are the primary antibodies that do not have specificity for any target. These can be used as negative control and are helpful in determining the background staining due to secondary antibodies. By compa...

Read More

Thank you for contacting us. We can provide blocking peptide for ab47329 as a special request. Could you please confirm if you are interested in buying this? We unfortunately will not be able to supply the blocking peptide for ab18. I suggest t...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa cells)
Specification HeLa cells
Fixative Paraformaldehyde
Permeabilization Yes - 0.05% Triton X 100 in PBS for 5 min
Blocking step goat serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Sep 29 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - other (HeLa cells)
Loading amount 20 µg
Specification HeLa cells
Gel Running Conditions Reduced Denaturing (8% Tris Glycine)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Aug 18 2010

We do not have any report that this or any other Cdc's are androgen regulated. We are sorry that we do not have any further information and suggest you refer to the latest literature in the field.

This antibody has not yet been tested for cross-reactivity with rabbit.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"