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RabMAb

Anti-CDK1 + Cdk2 + Cdk3 + Cdk5 抗体 [E53] (ab32030)

製品の概要

  • 製品名
    Anti-CDK1 + Cdk2 + Cdk3 + Cdk5 antibody [E53]
  • 製品の詳細
    Rabbit monoclonal [E53] to CDK1 + Cdk2 + Cdk3 + Cdk5
  • アプリケーション
    適用あり: WB, IP, ICC/IFmore details
    適用なし: Flow Cyt or IHC
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide corresponding to Human CDK1 aa 1-100.

  • ポジティブ・コントロール
    • WB: Hela cell lysate.
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    A trial size is available to purchase for this antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab32030 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/2000 - 1/10000. Detects a band of approximately 35 kDa (predicted molecular weight: 34 kDa).
IP 1/40.
ICC/IF Use at an assay dependent concentration.
  • 追加情報
    Is unsuitable for Flow Cyt or IHC.
  • ターゲット情報

    画像

    • All lanes : Anti-CDK1 + Cdk2 + Cdk3 + Cdk5 antibody [E53] (ab32030) at 1/10000 dilution

      Lane 1 : Empty vector transfected 293T (human embryonic kidney) whole cell lysates
      Lane 2 : CDK2-GFP transfected 293T (human embryonic kidney) whole cell lysates
      Lane 3 : CDK3-GFP transfected 293T (human embryonic kidney) whole cell lysates

      Lysates/proteins at 15 µg per lane.

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size : 34 kDa
      Observed band size : 62 kDa (why is the actual band size different from the predicted?)


      Exposure time : 1 second

      Blocking/Diluting buffer 5% NFDM/TBST

      The endogenous CDK is detected at higher exposure.

      We are not sure the bands around 35kDa. Perhaps they are degradation products.

    • Immunocytochemistry/ Immunofluorescence analysis of HeLa cells labeling CDK1 + Cdk2 + Cdk3 + Cdk5 with ab32030 at 1/200 dilution. The cells were fixed with paraformaldehyde and permeabilized with 0.5% Triton X-100 in PBS. Staining with ab32030 at 1/200 was carried out in PBS buffer for 1 hour at 22°C. DAPI was used to counterstain. ab150081, a Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed secondary antibody was used at 1/200 dilution.
      Under these conditions, the antibody produced a strong, nuclear-enriched focal staining pattern in interphase nuclei.

      See Abreview

    • All lanes : Anti-CDK1 + Cdk2 + Cdk3 + Cdk5 antibody [E53] (ab32030) at 1/2000 dilution

      Lane 1 : Empty vector transfected 293T (human embryonic kidney) whole cell lysates
      Lane 2 : CDK1-GFP transfected 293T (human embryonic kidney) whole cell lysates
      Lane 3 : CDK5-GFP transfected 293T (human embryonic kidney) whole cell lysates

      Lysates/proteins at 15 µg per lane.

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size : 34 kDa


      Exposure time : 15 seconds

      Blocking/Diluting buffer 5% NFDM/TBST

      34kDa band represents endogenous CDK, and 62kDa represents CDK-GFP.

    • Anti-CDK1 + Cdk2 + Cdk3 + Cdk5 antibody [E53] (ab32030) at 1/5000 dilution + Hela cell lysate

      Predicted band size : 34 kDa
      Observed band size : 35 kDa (why is the actual band size different from the predicted?)

    参考文献

    ab32030 has not yet been referenced specifically in any publications.

    レビューと Q&A

    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HeLa)
    Permeabilization
    Yes - 0.5% Triton X-100 in PBS
    Specification
    HeLa
    Fixative
    Paraformaldehyde
    Username

    Dr. Kirk Mcmanus

    Verified customer

    投稿 Jan 16 2017

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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