Anti-CDK1 + Cdk2 + Cdk3 + Cdk5 抗体 [E53] (ab32030)

製品の概要

  • 製品名Anti-CDK1 + Cdk2 + Cdk3 + Cdk5 antibody [E53]
  • 製品の詳細
    Rabbit monoclonal [E53] to CDK1 + Cdk2 + Cdk3 + Cdk5
  • アプリケーション適用あり: WB, IP, ICC/IFmore details
    適用なし: Flow Cyt or IHC
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human CDK1 aa 1-100.

  • ポジティブ・コントロール
    • WB: Hela cell lysate.
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    A trial size is available to purchase for this antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab32030 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/2000 - 1/10000. Detects a band of approximately 35 kDa (predicted molecular weight: 34 kDa).
IP 1/40.
ICC/IF Use at an assay dependent concentration.
  • 追加情報Is unsuitable for Flow Cyt or IHC.
  • ターゲット情報

    Anti-CDK1 + Cdk2 + Cdk3 + Cdk5 antibody [E53] 画像

    • All lanes : Anti-CDK1 + Cdk2 + Cdk3 + Cdk5 antibody [E53] (ab32030) at 1/10000 dilution

      Lane 1 : Empty vector transfected 293T (human embryonic kidney) whole cell lysates
      Lane 2 : CDK2-GFP transfected 293T (human embryonic kidney) whole cell lysates
      Lane 3 : CDK3-GFP transfected 293T (human embryonic kidney) whole cell lysates

      Lysates/proteins at 15 µg per lane.

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size : 34 kDa
      Observed band size : 62 kDa (why is the actual band size different from the predicted?)


      Exposure time : 1 second

      Blocking/Diluting buffer 5% NFDM/TBST

      The endogenous CDK is detected at higher exposure.

      We are not sure the bands around 35kDa. Perhaps they are degradation products.

    • Immunocytochemistry/ Immunofluorescence analysis of HeLa cells labeling CDK1 + Cdk2 + Cdk3 + Cdk5 with ab32030 at 1/200 dilution. The cells were fixed with paraformaldehyde and permeabilized with 0.5% Triton X-100 in PBS. Staining with ab32030 at 1/200 was carried out in PBS buffer for 1 hour at 22°C. DAPI was used to counterstain. ab150081, a Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed secondary antibody was used at 1/200 dilution.
      Under these conditions, the antibody produced a strong, nuclear-enriched focal staining pattern in interphase nuclei.

      See Abreview

    • All lanes : Anti-CDK1 + Cdk2 + Cdk3 + Cdk5 antibody [E53] (ab32030) at 1/2000 dilution

      Lane 1 : Empty vector transfected 293T (human embryonic kidney) whole cell lysates
      Lane 2 : CDK1-GFP transfected 293T (human embryonic kidney) whole cell lysates
      Lane 3 : CDK5-GFP transfected 293T (human embryonic kidney) whole cell lysates

      Lysates/proteins at 15 µg per lane.

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size : 34 kDa


      Exposure time : 15 seconds

      Blocking/Diluting buffer 5% NFDM/TBST

      34kDa band represents endogenous CDK, and 62kDa represents CDK-GFP.

    • Anti-CDK1 + Cdk2 + Cdk3 + Cdk5 antibody [E53] (ab32030) at 1/5000 dilution + Hela cell lysate

      Predicted band size : 34 kDa
      Observed band size : 35 kDa (why is the actual band size different from the predicted?)

    Anti-CDK1 + Cdk2 + Cdk3 + Cdk5 antibody [E53] (ab32030) 使用論文

    ab32030 has not yet been referenced specifically in any publications.

    Product Wall

    Application Immunocytochemistry/ Immunofluorescence
    Sample Human Cell (HeLa)
    Permeabilization Yes - 0.5% Triton X-100 in PBS
    Specification HeLa
    Fixative Paraformaldehyde
    Username

    Dr. Kirk McManus

    Verified customer

    投稿 Jan 16 2017

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"