Anti-CDK1 + CDK2 + CDK3 + CDK5 抗体 [E53] (ab32030)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [E53] to CDK1 + CDK2 + CDK3 + CDK5
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-CDK1 + CDK2 + CDK3 + CDK5 antibody [E53]
CDK1+CDK2+CDK3+CDK5 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [E53] to CDK1 + CDK2 + CDK3 + CDK5 -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details
適用なし: Flow Cyt or IHC -
種交差性
交差種: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- WB: Hela cell lysate.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
E53 -
アイソタイプ
IgG -
研究分野
関連製品
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab32030の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/2000 - 1/10000. Detects a band of approximately 35 kDa (predicted molecular weight: 34 kDa).
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特記事項 |
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WB
1/2000 - 1/10000. Detects a band of approximately 35 kDa (predicted molecular weight: 34 kDa). |
ターゲット情報
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細胞内局在
CDK1: Nucleus. CDK5: Cytoplasm. Cell projection > lamellipodium. Cell projection > growth cone. In axonal growth cone with extension to the peripheral lamellipodia. -
参照データベース
- Entrez Gene: 1017 Human
- Entrez Gene: 1018 Human
- Entrez Gene: 1020 Human
- Entrez Gene: 983 Human
- Omim: 116940 Human
- Omim: 116953 Human
- Omim: 123828 Human
- Omim: 123831 Human
see all -
製品の状態
CDK1: CDK1 can be located to the Nucleus, cytoplasm and Mithocondria. It's cytoplasmic during interphase and reversibly translocated from cytoplasm to the nucleus when phosphorilated before G2-M transition when associated with cyclin-B1. Accumulates in mitochondria in G2-arrested cells upon DNA-damage.
画像
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All lanes : Anti-CDK1 + CDK2 + CDK3 + CDK5 antibody [E53] (ab32030) at 1/10000 dilution
Lane 1 : Empty vector transfected 293T (human embryonic kidney) whole cell lysates
Lane 2 : CDK2-GFP transfected 293T (human embryonic kidney) whole cell lysates
Lane 3 : CDK3-GFP transfected 293T (human embryonic kidney) whole cell lysates
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 34 kDa
Observed band size: 62 kDa why is the actual band size different from the predicted?
Exposure time: 1 secondBlocking/Diluting buffer 5% NFDM/TBST
The endogenous CDK is detected at higher exposure.
We are not sure the bands around 35kDa. Perhaps they are degradation products.
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All lanes : Anti-CDK1 + CDK2 + CDK3 + CDK5 antibody [E53] (ab32030) at 1/2000 dilution
Lane 1 : Empty vector transfected 293T (human embryonic kidney) whole cell lysates
Lane 2 : CDK1-GFP transfected 293T (human embryonic kidney) whole cell lysates
Lane 3 : CDK5-GFP transfected 293T (human embryonic kidney) whole cell lysates
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 34 kDa
Exposure time: 15 secondsBlocking/Diluting buffer 5% NFDM/TBST
34kDa band represents endogenous CDK, and 62kDa represents CDK-GFP.
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Anti-CDK1 + CDK2 + CDK3 + CDK5 antibody [E53] (ab32030) at 1/5000 dilution + HeLa cell lysate
Predicted band size: 34 kDa
Observed band size: 35 kDa why is the actual band size different from the predicted?
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab32030 は 1 報の論文で使用されています。
- Ratovitski EA Phospho-?Np63a-dependent microRNAs modulate chemoresistance of squamous cell carcinoma cells to cisplatin: at the crossroads of cell life and death. FEBS Lett 587:2536-41 (2013). PubMed: 23831023