製品の概要

  • 製品名Anti-CDCA7L antibody
    CDCA7L 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to CDCA7L
  • アプリケーション適用あり: WB, IPmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide corresponding to a region between residue 400 and the C-terminus (residue 454) of Human CDCA7L (NP_061189.2)

  • ポジティブ・コントロール
    • Whole cell lysates from HeLa and 293T cells.

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab70637 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/2000 - 1/10000. Detects a band of approximately 53 kDa (predicted molecular weight: 53 kDa).
IP Use at 2-5 µg/mg of lysate.

ターゲット情報

  • 関連性CDCA7L is a member of the CDCA (cell division cycle associated) protein family. Members of this family have expression patterns associated with other known cell cycle genes such as cyclins and CDC genes. CDCA7L plays a role in transcriptional regulation as a repressor that inhibits monoamine oxidase A (MAOA) activity and gene expression by binding to the promoter. It also plays an important oncogenic role in mediating the full transforming effect of MYC in medulloblastoma cells. It is involved in apoptotic signaling pathways and may act downstream of P38-kinase and BCL-2, but upstream of CASP3/caspase-3 as well as CCND1/cyclin D1 and E2F1.
  • 細胞内局在Cytoplasm. Nucleus. Note: associates with chromatin. Translocates from cytoplasm to nucleus under dexamethasone induction.
  • 参照データベース
  • 別名
    • c Myc Interacting protein JPO2 antibody
    • CDCA7L antibody
    • DKFZp762L0311 antibody
    • HR1 antibody
    • JPO2 antibody
    • Protein JPO2 antibody
    • R1 antibody
    • RAM2 antibody
    • Transcription Factor RAM2 antibody
    see all

Anti-CDCA7L antibody 画像

  • All lanes : Anti-CDCA7L antibody (ab70637) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg

    Developed using the ECL technique

    Predicted band size : 53 kDa
    Observed band size : 53 kDa
    Additional bands at : 110 kDa,130 kDa,70 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 30 seconds
  • Detection of CDCA7L by Western Blot of Immunprecipitate. ab70637 at 1µg/ml staining CDCA7L in HeLa whole cell lysate immunoprecipitated using ab70637 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: chemiluminescence with exposure time of 30 seconds.

Anti-CDCA7L antibody (ab70637) 使用論文

ab70637 has not yet been referenced specifically in any publications.

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