The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2000 - 1/10000. Detects a band of approximately 53 kDa (predicted molecular weight: 53 kDa).
Use at 2-5 µg/mg of lysate.
CDCA7L is a member of the CDCA (cell division cycle associated) protein family. Members of this family have expression patterns associated with other known cell cycle genes such as cyclins and CDC genes. CDCA7L plays a role in transcriptional regulation as a repressor that inhibits monoamine oxidase A (MAOA) activity and gene expression by binding to the promoter. It also plays an important oncogenic role in mediating the full transforming effect of MYC in medulloblastoma cells. It is involved in apoptotic signaling pathways and may act downstream of P38-kinase and BCL-2, but upstream of CASP3/caspase-3 as well as CCND1/cyclin D1 and E2F1.
Note: associates with chromatin. Translocates from cytoplasm to nucleus under dexamethasone induction.
All lanes : Anti-CDCA7L antibody (ab70637) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg Lane 2 : HeLa whole cell lysate at 15 µg Lane 3 : HeLa whole cell lysate at 5 µg Lane 4 : 293T whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 53 kDa Observed band size: 53 kDa Additional bands at: 110 kDa, 130 kDa, 70 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 30 seconds
Immunoprecipitation - CDCA7L antibody (ab70637)
Detection of CDCA7L by Western Blot of Immunprecipitate.
ab70637 at 1µg/ml staining CDCA7L in HeLa whole cell lysate immunoprecipitated using ab70637 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: chemiluminescence with exposure time of 30 seconds.