製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to CDCA5
  • アプリケーション
    適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Recombinant fragment
    交差が予測される動物種: Human
  • 免疫原

    Synthetic peptide corresponding to the N terminal of Human CDCA5.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab56054 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 28 kDa. This antibody has been tested in Western blot against the recombinant peptide used as an immunogen. We have no data on detection of endogenous protein.
ELISA Use at an assay dependent dilution.

ターゲット情報

  • 機能
    Regulator of sister chromatid cohesion in mitosis stabilizing cohesin complex association with chromatin. May antagonize the action of WAPAL which stimulates cohesin dissociation from chromatin. Cohesion ensures that chromosome partitioning is accurate in both meiotic and mitotic cells and plays an important role in DNA repair. Required for efficient DNA double-stranded break repair.
  • 配列類似性
    Belongs to the sororin family.
  • ドメイン
    The KEN box is required for the association with the APC/C complex.
  • 翻訳後修飾
    Phosphorylated. Phosphorylation, as cells enter mitosis, disrupts the interaction with PDS5A and relieves the inhibition of WAPAL by CDCA5.
    Ubiquitinated by the APC/C complex in G1, leading to its degradation.
  • 細胞内局在
    Nucleus. Chromosome. Cytoplasm. Associates with nuclear chromatin from S phase until metaphase and is released in the cytoplasm upon nuclear envelope breakdown.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CDCA5 antibody
    • CDCA5_HUMAN antibody
    • Cell division cycle associated protein 5 antibody
    • Cell division cycle-associated protein 5 antibody
    • MGC16386 antibody
    • p35 antibody
    • Sororin antibody
    see all

プロトコール

Anti-CDCA5 antibody (ab56054) 使用論文

ab56054 has not yet been referenced specifically in any publications.

Product Wall

Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (Human osteosarcoma U2OS cells)
Loading amount
90 µg
Specification
Human osteosarcoma U2OS cells
Gel Running Conditions
Reduced Denaturing (4-18% gel)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Jun 13 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

登録