製品の概要

  • 製品名Anti-CDCA5 antibody
    CDCA5 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to CDCA5
  • アプリケーション適用あり: WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Rabbit, Cow, Cat, Dog, Pig
  • 免疫原

    Synthetic peptide corresponding to a region within the internal sequence amino acids 143-192 (RRSYSRLETL GSASTSTPGR RSCFGFEGLL GAEDLSGVSP VVCSKLTEVP) of Human CDCA5 (NP_542399).

  • ポジティブ・コントロール
    • OVCAR3 cell lysate

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab104780 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 28 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

ターゲット情報

  • 機能Regulator of sister chromatid cohesion in mitosis stabilizing cohesin complex association with chromatin. May antagonize the action of WAPAL which stimulates cohesin dissociation from chromatin. Cohesion ensures that chromosome partitioning is accurate in both meiotic and mitotic cells and plays an important role in DNA repair. Required for efficient DNA double-stranded break repair.
  • 配列類似性Belongs to the sororin family.
  • ドメインThe KEN box is required for the association with the APC/C complex.
  • 翻訳後修飾Phosphorylated. Phosphorylation, as cells enter mitosis, disrupts the interaction with PDS5A and relieves the inhibition of WAPAL by CDCA5.
    Ubiquitinated by the APC/C complex in G1, leading to its degradation.
  • 細胞内局在Nucleus. Chromosome. Cytoplasm. Associates with nuclear chromatin from S phase until metaphase and is released in the cytoplasm upon nuclear envelope breakdown.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CDCA5 antibody
    • CDCA5_HUMAN antibody
    • Cell division cycle associated protein 5 antibody
    • Cell division cycle-associated protein 5 antibody
    • MGC16386 antibody
    • p35 antibody
    • Sororin antibody
    see all

Anti-CDCA5 antibody 画像

  • Anti-CDCA5 antibody (ab104780) at 1 µg/ml + OVCAR3 cell lyate at 10 µg

    Predicted band size : 28 kDa

Anti-CDCA5 antibody (ab104780) 使用論文

ab104780 has not yet been referenced specifically in any publications.

Product Wall

Abreviews
Application Western blot
Sample Human Cell lysate - whole cell (Human osteosarcoma U2OS cells)
Loading amount 90 µg
Specification Human osteosarcoma U2OS cells
Gel Running Conditions Reduced Denaturing (4-18% gel)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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投稿 Jun 13 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"