製品の概要

  • 製品名Anti-CDCA2 antibody
    CDCA2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to CDCA2
  • アプリケーション適用あり: WB, ICC/IFmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 950 to the C-terminus of Human CDCA2.

    (Peptide available as ab45267.)

  • ポジティブ・コントロール
    • This antibody gave a positive control in the following human lysates: Hela nuclear HepG2 nuclear Jurkat nuclear (data not shown)

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab45129 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 125 kDa (predicted molecular weight: 113 kDa).
ICC/IF Use a concentration of 5 µg/ml.

ターゲット情報

  • 関連性CDCA2 is a regulator of chromosome structure during mitosis. CDCA2 forms an essential complex with protein phosphatase PP1-gamma subunit 1 (PPP1CC) and is required for the recruitment of PP1 to chromatin. Knockdown of CDCA2 by RNAi causes large-scale cell death by apoptosis, as does overexpression of a dominant-negative mutant of the protein.
  • 細胞内局在Nuclear
  • 参照データベース
  • 別名
    • cell division cycle associated 2 antibody
    • PPP1R81 antibody
    • protein phosphatase 1 [gamma] (PPP1R3A) binding protein Repo-Man antibody
    • protein phosphatase 1, regulatory subunit 81 antibody
    • Recruits PP1 onto mitotic chromatin at anaphase protein antibody
    • Repo Man antibody
    • Repo-Man antibody
    see all

Anti-CDCA2 antibody 画像

  • All lanes : Anti-CDCA2 antibody (ab45129) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate
    Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 4 : HepG2 nuclear extract lysate (ab14660)

    Lysates/proteins at 10 µg per lane.

    Secondary
    IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size : 113 kDa
    Observed band size : 125 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 40 kDa. We are unsure as to the identity of these extra bands.
  • ICC/IF image of ab45129 stained HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab45129, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-CDCA2 antibody (ab45129) 使用論文

This product has been referenced in:
  • Wurzenberger C  et al. Sds22 and Repo-Man stabilize chromosome segregation by counteracting Aurora B on anaphase kinetochores. J Cell Biol 198:173-83 (2012). Read more (PubMed: 22801782) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HeLa, HEK293, U2OS)
Loading amount 30 µg
Specification HeLa, HEK293, U2OS
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
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投稿 Oct 08 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"