製品の概要

  • 製品名
    Anti-Cdc7 Kinase antibody [DCS-341]
    Cdc7 Kinase 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [DCS-341] to Cdc7 Kinase
  • アプリケーション
    適用あり: ELISA, WB, IP, ICCmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Full length protein (Human).

  • ポジティブ・コントロール
    • A431 whole cell lysate.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab10535 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA Use at an assay dependent dilution.
WB Use a concentration of 0.4 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 64 kDa).
IP Use at an assay dependent dilution.
ICC Use at an assay dependent dilution.

ターゲット情報

  • 機能
    Seems to phosphorylate critical substrates that regulate the G1/S phase transition and/or DNA replication. Can phosphorylates MCM2 and MCM3.
  • 配列類似性
    Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDC7 subfamily.
    Contains 1 protein kinase domain.
  • 細胞内局在
    Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Cdc 7 antibody
    • CDC7 antibody
    • CDC7 cell division cycle 7 antibody
    • CDC7 cell division cycle 7 like 1 antibody
    • CDC7 L1 antibody
    • Cdc7 like 1 antibody
    • CDC7 related kinase antibody
    • CDC7-related kinase antibody
    • CDC7_HUMAN antibody
    • CDC7L 1 antibody
    • CDC7L1 antibody
    • Cell division cycle 7 homolog antibody
    • Cell division cycle 7 kinase antibody
    • Cell division cycle 7 like protein 1 antibody
    • Cell division cycle 7 related protein kinase antibody
    • Cell division cycle 7-related protein kinase antibody
    • HsCDC 7 antibody
    • HsCdc7 antibody
    • Hsk 1 antibody
    • Hsk1 antibody
    • HuCDC 7 antibody
    • huCdc7 antibody
    • MGC117361 antibody
    • MGC126237 antibody
    • MGC126238 antibody
    see all

Anti-Cdc7 Kinase antibody [DCS-341] 画像

  • All lanes : Anti-Cdc7 Kinase antibody [DCS-341] (ab10535) at 1/1000 dilution

    Lane 1 : U2OS Cell lysate with siRNA control (scramble siRNA) siCdc7
    Lane 2 : U2OS Cell Lysate with siRNA against Cdc7

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat anti-Mouse monoclonal conjugated to HRP at 1/1000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 64 kDa

    This image is courtesy of an Abreview submitted by Remi Buisson

    Blocking Step: 1 hour at room temperature with 5% Milk in TBS.

    Diluent: 5% Milk in TBS.

    See Abreview



  • Predicted band size : 64 kDa

    Image from Heffernan TP et al, J Biol Chem. 2007 Mar 30;282(13):9458-68. Epub 2007 Feb 2, Fig 7, DOI 10.1074/jbc.M611292200

    FLAG-Dbf4 forms a complex with endogenous Cdc7 following DNA damage. HeLa cells were transiently transfected with a FLAG-Dbf4 expression vector. 48 hours later cells were treated with 8 J/m2 UVC, 10 µm etoposide, or 2 mm HU. Cells were harvested 1 hour later and FLAG-Dbf4 immunoprecipitated with anti-FLAG-agarose (NS corresponds to immunoprecipitation with normal mouse IgG). The effect of DNA damage on Cdc7-Dbf4 complex formation was measured by Western immunoblot analysis using ab10535.

Anti-Cdc7 Kinase antibody [DCS-341] (ab10535) 使用論文

This product has been referenced in:
  • Suzuki T  et al. Inhibition of DNA damage-induced apoptosis through Cdc7-mediated stabilization of Tob. J Biol Chem 287:40256-65 (2012). ICC/IF, Dot blot ; Human . Read more (PubMed: 23066029) »
  • Petermann E  et al. Chk1 promotes replication fork progression by controlling replication initiation. Proc Natl Acad Sci U S A 107:16090-5 (2010). WB ; Human . Read more (PubMed: 20805465) »

See all 4 Publications for this product

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Application
Western blot
Loading amount
10 µg
Gel Running Conditions
Reduced Denaturing (10 %)
Sample
Human Cell lysate - whole cell (U2OS)
Specification
U2OS
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Dr. Remi Buisson

Verified customer

投稿 Oct 31 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (HEK293T)
Loading amount
50 µg
Specification
HEK293T
Treatment
Cdc7 siRNA
Gel Running Conditions
Reduced Denaturing (8% SDS-PAGE)
Blocking step
Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Abcam user community

Verified customer

投稿 Jun 01 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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