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RabMAb

Anti-Cdc6 (phospho S54) 抗体 [EPR759Y] (ab75809)

製品の概要

  • 製品名
    Anti-Cdc6 (phospho S54) antibody [EPR759Y]
    Cdc6 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR759Y] to Cdc6 (phospho S54)
  • 特異性
    ab75809 recognises Cdc6 only when phosphorylated at Serine 54.
  • アプリケーション
    適用あり: WB, ICC/IFmore details
    適用なし: Flow Cyt,IHC-P or IP
  • 種交差性
    交差種: Human
  • 免疫原

    A phospho-specific peptide corresponding to residues surrounding serine 54 of human Cdc6

  • ポジティブ・コントロール
    • Raji + FBS cell lysates. HeLa cells.
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab75809 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/2000. Detects a band of approximately 63 kDa (predicted molecular weight: 63 kDa).
ICC/IF 1/100 - 1/250.
  • 追加情報
    Is unsuitable for Flow Cyt,IHC-P or IP.
  • ターゲット情報

    • 機能
      Involved in the initiation of DNA replication. Also participates in checkpoint controls that ensure DNA replication is completed before mitosis is initiated.
    • 配列類似性
      Belongs to the CDC6/cdc18 family.
    • 細胞内局在
      Nucleus. Cytoplasm. The protein is nuclear in G1 and cytoplasmic in S-phase cells.
    • Information by UniProt
    • 参照データベース
    • 別名
      • Cdc 18L antibody
      • Cdc 6 antibody
      • CDC18 (cell division cycle 18, S.pombe, homolog) like antibody
      • CDC18 (S.pombe) antibody
      • Cdc18 related protein antibody
      • CDC18(S.pombe) antibody
      • Cdc18-related protein antibody
      • Cdc18L antibody
      • cdc6 antibody
      • CDC6 cell division cycle 6 homolog antibody
      • CDC6 related protein antibody
      • CDC6-related protein antibody
      • CDC6_HUMAN antibody
      • Cdc6p antibody
      • CELL CYCLE CONTROLLER CDC6 antibody
      • Cell division control protein 6 antibody
      • Cell division control protein 6 homolog antibody
      • Cell division cycle 6 homolog (S. cerevisiae) antibody
      • Cell division cycle 6 homolog antibody
      • Cell division cycle 6, S. cerevisiae, homolog of antibody
      • HsCDC 6 antibody
      • HsCDC18 antibody
      • HsCDC6 antibody
      • p62 antibody
      • p62(cdc 6) antibody
      • p62(cdc6) antibody
      see all

    Anti-Cdc6 (phospho S54) antibody [EPR759Y] 画像

    • Primary ab dilution 1:1000 dilution. Secondary ab description and code (ab id) Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051). Secondary ab dilution 1:100,000 dilution. Blocking buffer and concentration 5% NFDM/TBST. Diluting buffer and concentration 5% NFDM /TBST. Lane 1:Cdc6 (pS54) phospho peptide, Lane 2:Cdc6 non-phospho peptide. Exposure time 3 minutes

    • Cell line 293T (Human embryonic kidney epithelial cell). Target AbID Ab75809 anti- Cdc6 (phospho S54)

      ab150077 AlexaFluor®488 Goat anti-Rabbit secondary. Counterstain AbID Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594). Fixative 4% Paraformaldehyde. Permeabilisation 0.1% tritonX-100. Nuclear counter stain DAPI. Datasheet Comments Confocal image showing the expression was increased after treatment with Hydroxyurea (3mM 20hour), the signal was decreased after treatment with the Lambda Protein Phosphatase 31℃ for 2h. Target 1oAb dilution 1:100 2.4 μg/ml, Target 2ndry Ab dilution 1:1000 2 μg/ml, Counterstain Ab dilution 1:200 2.5 μg/ml

    • All lanes : Anti-Cdc6 (phospho S54) antibody [EPR759Y] (ab75809) at 1/5000 dilution

      Lane 1 : Hela (Human cervix adenocarcinoma epithelial cell) whole cell lysates
      Lane 2 : Hela (Human cervix adenocarcinoma epithelial cell) treated with Hydroxyurea (3mM, 20 h). Whole cell lysates
      Lane 3 : Hela (Human cervix adenocarcinoma epithelial cell) treated with Hydroxyurea (3mM, 20 h). Whole cell lysates.Then the membrane was incubated with phosphatase.

      Lysates/proteins at 15 µg per lane.

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size : 63 kDa


      Exposure time : 30 seconds

      Blocking buffer and diluting buffer: 5% NFDM/TBST

    • All lanes : Anti-Cdc6 (phospho S54) antibody [EPR759Y] (ab75809) at 1/2000 dilution

      Lane 1 : Raji cell lysate
      Lane 2 : Raji + FBS cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 63 kDa
      Observed band size : 63 kDa

    Anti-Cdc6 (phospho S54) antibody [EPR759Y] (ab75809) 使用論文

    This product has been referenced in:
    • Fan X  et al. Role of Cdc6 in re-replication in cells expressing human papillomavirus E7 oncogene. Carcinogenesis 37:799-809 (2016). Read more (PubMed: 27207654) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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