Anti-Cdc6 (phospho S106) 抗体 [EPR761(2)] (ab76422)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR761(2)] to Cdc6 (phospho S106)
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-Cdc6 (phospho S106) antibody [EPR761(2)]
Cdc6 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR761(2)] to Cdc6 (phospho S106) -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
(Peptide available asab197758) -
ポジティブ・コントロール
- WB: Raji whole cell lysate.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR761(2) -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Immunizing Peptide (Blocking)
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab76422の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/1000 - 1/2000. Detects a band of approximately 63 kDa (predicted molecular weight: 63 kDa).Can be blocked with Cdc6 (phospho S106) peptide (ab197758).
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特記事項 |
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WB
1/1000 - 1/2000. Detects a band of approximately 63 kDa (predicted molecular weight: 63 kDa).Can be blocked with Cdc6 (phospho S106) peptide (ab197758). |
ターゲット情報
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機能
Involved in the initiation of DNA replication. Also participates in checkpoint controls that ensure DNA replication is completed before mitosis is initiated. -
配列類似性
Belongs to the CDC6/cdc18 family. -
細胞内局在
Nucleus. Cytoplasm. The protein is nuclear in G1 and cytoplasmic in S-phase cells. - Information by UniProt
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参照データベース
- Entrez Gene: 990 Human
- Omim: 602627 Human
- SwissProt: Q99741 Human
- Unigene: 405958 Human
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別名
- Cdc 18L antibody
- Cdc 6 antibody
- CDC18 (cell division cycle 18, S.pombe, homolog) like antibody
see all
画像
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All lanes : Anti-Cdc6 (phospho S106) antibody [EPR761(2)] (ab76422) at 1/1000 dilution (Purified)
Lane 1 : Raji (Human Burkitt's lymphoma B lymphocyte) starved for 24 hours whole cell lysate
Lane 2 : Raji (Human Burkitt's lymphoma B lymphocyte) starved for 24 hours then treated with 10%FBS for 24 hours whole cell lysate
Lane 3 : Raji (Human Burkitt's lymphoma B lymphocyte) starved for 24 hours then treated with 10%FBS for 24 hours whole cell lysate, then the menbrane treated with Alkaline Phosphatase for 1 hour
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 63 kDa
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab76422 は 2 報の論文で使用されています。
- Iwai K et al. Molecular mechanism and potential target indication of TAK-931, a novel CDC7-selective inhibitor. Sci Adv 5:eaav3660 (2019). PubMed: 31131319
- Betts JF et al. D-amino acid oxidase is expressed in the ventral tegmental area and modulates cortical dopamine. Front Synaptic Neurosci 6:11 (2014). IF . PubMed: 24822045