The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 4 - 8 µg/ml.
Use a concentration of 1 µg/ml. Predicted molecular weight: 92 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
機能DNA-binding protein involved in cell cycle control. May act as a transcription activator. Component of the PRP19-CDC5L complex that forms an integral part of the spliceosome and is required for activating pre-mRNA splicing.
組織特異性Ubiquitously expressed in both fetal and adult tissues.
関連疾患A chromosomal aberration involving CDC5L is found in multicystic renal dysplasia. Translocation t(6;19)(p21;q13.1) with USF2.
配列類似性Belongs to the CEF1 family. Contains 2 HTH myb-type DNA-binding domains.
翻訳後修飾Phosphorylated on serine and threonine residues. Phosphorylation on Thr-411 and Thr-438 is required for CDC5L-mediated mRNA splicing. Has no effect on subcellular location nor on homodimerization. Phosphorylated in vitro by CDK2. Phosphorylation enhances interaction with PPP1R8.
細胞内局在Nucleus. Nucleus speckle. Cytoplasm. May shuttle between cytoplasm and nucleus.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung tissue labelling CDC5L with ab86971 at 4.0-8.0µg/ml. Positive staining shown in alveolar cells. Magnification: 400X.
Western blot - CDC5L antibody (ab86971)
Anti-CDC5L antibody (ab86971) at 1 µg/ml (in 5% skim milk / PBS buffer) + 721_B cell lysate at 10 µg
Secondary HRP conjugated anti-Rabbit IgG at 1/50000 dilution