製品の概要

  • 製品名
    Anti-CDC42SE2 antibody
  • 製品の詳細
    Rabbit polyclonal to CDC42SE2
  • アプリケーション
    適用あり: IHC-P, WB, ICC/IFmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    antigen sequence: EPTNFVHTAH VGSGDLFSGM NSVSSIQNQM QSKGGYGGGM PANVQMQLVD TKAG, corresponding to amino acids 31-84 of Human CDC42SE2.

  • ポジティブ・コントロール
    • RT 4, U 251 MG, Human Liver and Human Tonsil lysates; Human small intestine tissue; A431 cells.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at -20°C or -80°C. Avoid freeze / thaw cycle. Store undiluted.
  • バッファー
    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab122492 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/250 - 1/500.
ICC/IF Use a concentration of 1 - 4 µg/ml. Fixation/Permeabilization: PFA/Triton X-100

ターゲット情報

  • 機能
    Probably involved in the organization of the actin cytoskeleton by acting downstream of CDC42, inducing actin filament assembly. Alters CDC42-induced cell shape changes. In activated T-cells, may play a role in CDC42-mediated F-actin accumulation at the immunological synapse. May play a role in early contractile events in phagocytosis in macrophages.
  • 組織特異性
    Widely expressed. Expressed at higher level in T lymphocytes. Highly expressed in CCRF-CEM T lymphocytes, Jurkat T lymphocytes, and Raji B lymphocytes compared (at protein level).
  • 配列類似性
    Belongs to the CDC42SE/SPEC family.
    Contains 1 CRIB domain.
  • ドメイン
    The CRIB domain mediates interaction with CDC42.
  • 細胞内局在
    Cytoplasm > cytoskeleton. Cell membrane. Cell projection > phagocytic cup. Recruited to the activated TCR prior actin polymerization. Localizes at the phagocytic cup of macrophages.
  • Information by UniProt
  • 参照データベース
  • 別名
    • C42S2_HUMAN antibody
    • CDC42 small effector protein 2 antibody
    • Cdc42se2 antibody
    • FLJ21967 antibody
    • Small effector of CDC42 protein 2 antibody
    • SPEC2 antibody
    see all

画像

  • All lanes : Anti-CDC42SE2 antibody (ab122492) at 1/250 dilution

    Lane 1 : RT 4 cell lysate
    Lane 2 : U 251 MG cell lysate
    Lane 3 : Human Plasma lysate
    Lane 4 : Human Liver lysate
    Lane 5 : Human Tonsil lysate

    Developed using the ECL technique
  • ab122492 at 1/50 dilution staining CDC42SE2 in Paraffin-embedded Human small intestine tissue by Immunohistochemistry.
  • ab122492 at 4 µg/ml staining CDC42SE2 in PFA/Triton X-100 fixed/permeabilized A431 cells by Immunofluorescence (green).

参考文献

ab122492 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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