Anti-CDC42SE2 抗体 (ab122492)
Key features and details
- Rabbit polyclonal to CDC42SE2
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-CDC42SE2 antibody -
製品の詳細
Rabbit polyclonal to CDC42SE2 -
由来種
Rabbit -
アプリケーション
適用あり: IHC-P, WB, ICC/IFmore details -
種交差性
交差種: Human -
免疫原
antigen sequence: EPTNFVHTAH VGSGDLFSGM NSVSSIQNQM QSKGGYGGGM PANVQMQLVD TKAG, corresponding to amino acids 31-84 of Human CDC42SE2.
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ポジティブ・コントロール
- RT 4, U 251 MG, Human Liver and Human Tonsil lysates; Human small intestine tissue; A431 cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C or -80°C. Avoid freeze / thaw cycle. Store undiluted. -
バッファー
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab122492の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-P |
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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WB |
Use a concentration of 0.04 - 0.4 µg/ml.
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ICC/IF |
Use a concentration of 1 - 4 µg/ml. Fixation/Permeabilization: PFA/Triton X-100
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特記事項 |
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IHC-P
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
Use a concentration of 0.04 - 0.4 µg/ml. |
ICC/IF
Use a concentration of 1 - 4 µg/ml. Fixation/Permeabilization: PFA/Triton X-100 |
ターゲット情報
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機能
Probably involved in the organization of the actin cytoskeleton by acting downstream of CDC42, inducing actin filament assembly. Alters CDC42-induced cell shape changes. In activated T-cells, may play a role in CDC42-mediated F-actin accumulation at the immunological synapse. May play a role in early contractile events in phagocytosis in macrophages. -
組織特異性
Widely expressed. Expressed at higher level in T lymphocytes. Highly expressed in CCRF-CEM T lymphocytes, Jurkat T lymphocytes, and Raji B lymphocytes compared (at protein level). -
配列類似性
Belongs to the CDC42SE/SPEC family.
Contains 1 CRIB domain. -
ドメイン
The CRIB domain mediates interaction with CDC42. -
細胞内局在
Cytoplasm > cytoskeleton. Cell membrane. Cell projection > phagocytic cup. Recruited to the activated TCR prior actin polymerization. Localizes at the phagocytic cup of macrophages. - Information by UniProt
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参照データベース
- Entrez Gene: 56990 Human
- SwissProt: Q9NRR3 Human
- Unigene: 508829 Human
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別名
- C42S2_HUMAN antibody
- CDC42 small effector protein 2 antibody
- Cdc42se2 antibody
see all
画像
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All lanes : Anti-CDC42SE2 antibody (ab122492) at 1/250 dilution
Lane 1 : RT 4 cell lysate
Lane 2 : U 251 MG cell lysate
Lane 3 : Human Plasma lysate
Lane 4 : Human Liver lysate
Lane 5 : Human Tonsil lysate
Developed using the ECL technique. -
ab122492 at 1/50 dilution staining CDC42SE2 in Paraffin-embedded Human small intestine tissue by Immunohistochemistry.
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ab122492 at 4 µg/ml staining CDC42SE2 in PFA/Triton X-100 fixed/permeabilized A431 cells by Immunofluorescence (green).
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab122492 は論文での使用が確認できていません。