The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at 2-5 µg/mg of lysate.
追加情報Is unsuitable for WB.
機能May act as a downstream effector of CDC42 in cytoskeletal reorganization. Contributes to the actomyosin contractility required for cell invasion, through the regulation of MYPT1 and thus MLC2 phosphorylation.
組織特異性Abundant in the heart, brain, skeletal muscle, kidney, and pancreas, with little or no expression in the lung and liver.
配列類似性Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. DMPK subfamily. Contains 1 AGC-kinase C-terminal domain. Contains 1 CNH domain. Contains 1 CRIB domain. Contains 1 PH domain. Contains 1 phorbol-ester/DAG-type zinc finger. Contains 1 protein kinase domain.
細胞内局在Cytoplasm. Displays a dispersed punctate distribution and concentrates along the cell periphery, especially at the leading edge and cell-cell junction. This concentration is PH-domain dependent.
Anti-CDC42 binding protein kinase alpha antibody 画像
Immunoprecipitation - CDC42 binding protein kinase alpha antibody (ab95880)
Detection of Human CDC42 binding protein kinase alpha by Immunoprecipitation, using ab95880 at 3µg/mg lysate. Image shows immunoprecipitated CDC42 binding protein kinase alpha detected with post IP WB, loading 20% of IP and using HeLa whole cell lysate at 1mg, with a95880 at 1 µg/ml in lane 1, an antibody recognising a downstream epitope in lane 2 and control IgG in lane 3. Detection: Chemiluminescence with an exposure time of 10 seconds.
Anti-CDC42 binding protein kinase alpha antibody (ab95880) 使用論文
has not yet been referenced specifically in any publications.