製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to CDC42
  • アプリケーション
    適用あり: ICC/IF, WB, IHC-Fr, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Rat, Human, Saccharomyces cerevisiae
    交差が予測される動物種: Pig, Chimpanzee, Zebrafish
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 100 to the C-terminus of Human CDC42.

    (Peptide available as ab66571.)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in the following Human Whole Cell Lysates: HeLa, Jurkat, HepG2, MCF7, U2OS, A431 (data not shown), HEK 293 (data not shown) and SHSY-5Y (data not shown)

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab64533 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 21 kDa (predicted molecular weight: 21 kDa).
IHC-Fr 1/100.
IHC-P Use at an assay dependent concentration.

ターゲット情報

  • 機能
    Plasma membrane-associated small GTPase which cycles between an active GTP-bound and an inactive GDP-bound state. In active state binds to a variety of effector proteins to regulate cellular responses. Involved in epithelial cell polarization processes. Causes the formation of thin, actin-rich surface projections called filopodia.
  • 配列類似性
    Belongs to the small GTPase superfamily. Rho family. CDC42 subfamily.
  • 翻訳後修飾
    AMPylation at Tyr-32 and Thr-35 are mediated by bacterial enzymes in case of infection by H.somnus and V.parahaemolyticus, respectively. AMPylation occurs in the effector region and leads to inactivation of the GTPase activity by preventing the interaction with downstream effectors, thereby inhibiting actin assembly in infected cells. It is unclear whether some human enzyme mediates AMPylation; FICD has such ability in vitro but additional experiments remain to be done to confirm results in vivo.
  • 細胞内局在
    Cell membrane.
  • Information by UniProt
  • 参照データベース
  • 製品の状態
    There are 2 isoforms produced by alternative splicing. Isoform 1 also known as: Brain; Isoform 2 also known as: Placental.
  • 別名
    • CDC42 antibody
    • CDC42_HUMAN antibody
    • CDC42Hs antibody
    • Cell division control protein 42 homolog antibody
    • Cell division cycle 42 (GTP binding protein 25kDa) antibody
    • Cell division cycle 42 antibody
    • dJ224A6.1.1 (cell division cycle 42 (GTP-binding protein, 25kD)) antibody
    • dJ224A6.1.2 (cell division cycle 42 (GTP-binding protein, 25kD)) antibody
    • G25K antibody
    • G25K GTP-binding protein antibody
    • Growth regulating protein antibody
    • GTP binding protein 25kDa antibody
    • Small GTP binding protein CDC42 antibody
    • TKS antibody
    see all

Anti-CDC42 antibody 画像



  • Predicted band size : 21 kDa

    Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: CDC42 knockout HAP1 cell lysate (20 µg)
    Lane 3: MCF7 cell lysate (20 µg)
    Lane 4: HepG2 cell lysate (20 µg)
    Lanes 1 - 4: Merged signal (red and green). Green – ab64533 observed at 20 kDa. Red - loading control, ab8245, observed at 37 kDa.

    Ab64533 was shown to recognize CDC42 when CDC42 knockout samples were used, along with additional cross-reactive bands. Wild-type and CDC42 knockout samples were subjected to SDS-PAGE. Ab64533 and ab8245 (loading control to GAPDH) were diluted at 1 µg/ml and 1/10 000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.

  • All lanes : Anti-CDC42 antibody (ab64533) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 4 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
    Lane 5 : U2OS (Human osteosarcoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 21 kDa
    Observed band size : 21 kDa
    Additional bands at : 52 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 3 minutes

  • Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 21 kDa


    Exposure time : 1 minute
  • ICC/IF image of ab64533 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab64533, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 Goat anti-Rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive result in 4% pfa fixed (10 min) HeLa cells at 5µg/ml.

Anti-CDC42 antibody (ab64533) 使用論文

This product has been referenced in:
  • Druso JE  et al. An Essential Role for Cdc42 in the Functioning of the Adult Mammary Gland. J Biol Chem 291:8886-95 (2016). Read more (PubMed: 26912661) »
  • Wang JR  et al. Signaling Cascades Governing Cdc42-Mediated Chondrogenic Differentiation and Mensenchymal Condensation. Genetics 202:1055-69 (2016). Read more (PubMed: 26739452) »

See all 10 Publications for this product

Product Wall

Application
Immunohistochemistry (Frozen sections)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Sample
Zebrafish Tissue sections (retina)
Specification
retina
Permeabilization
Yes - triton X
Fixative
Paraformaldehyde
Username

Dr. Ryan Macdonald

Verified customer

投稿 May 22 2014

The nucleotide binding sites of this protein correspond to amino acids 10-17, 57-61 and 115-118.
Since the immunogen of this antibody corresponds to amino acids xxxxxxx, I can confirm that this antibody will not be able to distinguish between GTP ...

Read More

ab41429 does not detect RhoA, we have tested this directly using recombinant RhoA protein versus recombinant CDC42 protein. However, we have not tested reactivity to Rac1 and therefore, we cannot comment on the cross-reactivity with Rac1 since the epit...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Brain)
Specification
Brain
Fixative
Paraformaldehyde
Permeabilization
Yes - saponin
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Jan 17 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Saccharomyces cerevisiae Cell lysate - whole cell (SPhi992 strain)
Loading amount
50 µg
Specification
SPhi992 strain
Gel Running Conditions
Reduced Denaturing (12% Bis-TRIS in MOPS buffer)
Blocking step
Odyssey Blocking Agent (Lycor system) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Feb 18 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (MPRO (mouse promyelocytic) cells)
Loading amount
50 µg
Specification
MPRO (mouse promyelocytic) cells
Gel Running Conditions
Reduced Denaturing (12% Bis-TRIS in MOPS buffer)
Blocking step
Odyssey Blocking Agent (Lycor system) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Feb 18 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (HEK293)
Loading amount
50 µg
Specification
HEK293
Gel Running Conditions
Reduced Denaturing (12% Bis-TRIS in MOPS buffer)
Blocking step
Odyssey Blocking Agent (Lycor system) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Feb 10 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (colon)
Specification
colon
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2%
Username

Abcam user community

Verified customer

投稿 Feb 05 2010

Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa, Jurkat)
Loading amount
500 µg
Specification
HeLa, Jurkat
Gel Running Conditions
Reduced Denaturing (12,5% acrylamide gel)
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 18°C
Username

Mrs. Merly Saare

Verified customer

投稿 Dec 07 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Specification
HeLa
Fixative
Formaldehyde
Permeabilization
Yes - 0.1% Triton x-100 in PBS
Blocking step
Milk as blocking agent for 40 minute(s) · Concentration: 10% · Temperature: 23°C
Username

Mrs. Merly Saare

Verified customer

投稿 Sep 14 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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