Anti-Cdc37 (phospho S13) 抗体 [EPR4879] (ab108360)

製品の概要

  • 製品名Anti-Cdc37 (phospho S13) antibody [EPR4879]
    Cdc37 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR4879] to Cdc37 (phospho S13)
  • 特異性ab108360 only detects Cdc37 phosphorylated at Serine 13.
  • アプリケーション適用あり: WB, IPmore details
    適用なし: ICC or IHC-P
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Cdc37 (phospho S13). The exact sequence is proprietary.

  • ポジティブ・コントロール
    • WB: Jurkat, HeLa (treated with fetal bovine serum and Calyculin A) and HEK293 whole cell lysates.
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Alternative versions available:

    Anti-Cdc37 (phospho S13) antibody (HRP) [EPR4879] (ab202364)

    Anti-Cdc37 (phospho S13) antibody (Biotin) [EPR4879] (ab202888)

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab108360 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/10000. Predicted molecular weight: 44 kDa.
IP 1/10 - 1/100.
  • 追加情報Is unsuitable for ICC or IHC-P.
  • ターゲット情報

    • 機能Co-chaperone that binds to numerous kinases and promotes their interaction with the Hsp90 complex, resulting in stabilization and promotion of their activity.
    • 配列類似性Belongs to the CDC37 family.
    • 翻訳後修飾Constitutively sumoylated by UBE2I.
    • 細胞内局在Cytoplasm.
    • Information by UniProt
    • 参照データベース
    • 別名
      • CDC 37 antibody
      • Cdc37 antibody
      • CDC37 cell division cycle 37 homolog antibody
      • CDC37 cell division cycle 37 S cerevisiae homolog antibody
      • CDC37 cell division cycle 37, S cerevisiae, homolog of antibody
      • Cdc37 homolog antibody
      • CDC37 protein antibody
      • CDC37_HUMAN antibody
      • CDC37A antibody
      • cell division cycle 37 antibody
      • Cell division cycle 37 homolog antibody
      • Hsp90 chaperone protein kinase targeting subunit antibody
      • Hsp90 chaperone protein kinase targeting subunit p50Cdc37 antibody
      • Hsp90 chaperone protein kinase-targeting subunit antibody
      • Hsp90 co chaperone Cdc37 antibody
      • Hsp90 co-chaperone Cdc37 antibody
      • p50 antibody
      • p50Cdc37 antibody
      • S cerevisiae hypothetical protein CDC37 antibody
      see all

    Anti-Cdc37 (phospho S13) antibody [EPR4879] 画像

    • All lanes : Anti-Cdc37 (phospho S13) antibody [EPR4879] (ab108360) at 1/5000 dilution

      Lane 1 : Untreated HeLa whole cell lysates
      Lane 2 : HeLa were starved for 24 hours, then cultured in medium with 20% fetal bovine serum (FBS) for 5 hours, and then treated with 100ng/ml Calyculin A for 30 min whole cell lysates
      Lane 3 : HeLa were starved for 24 hours, then cultured in medium with 20% fetal bovine serum (FBS) for 5 hours, and then treated with 100ng/ml Calyculin A for 30 min whole cell lysates, the membrane was then incubated with Alkaline phosphatase

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500000 dilution

      Predicted band size : 44 kDa
      Observed band size : 44 kDa


      Exposure time : 3 minutes

      Blocking buffer and concentration 2% BSA/TBST
      Diluting buffer and concentration 2% BSA/TBST

    • All lanes : Anti-Cdc37 (phospho S13) antibody [EPR4879] (ab108360) at 1/20000 dilution

      Lane 1 : HeLa whole cell lysate - treated with fetal bovine serum and Calyculin A
      Lane 2 : HeLa whole cell lysate - untreated

      Lysates/proteins at 10 µg per lane.

      Secondary
      Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

      Predicted band size : 44 kDa
      Observed band size : 44 kDa


      Exposure time : 3 minutes

      Blocking and dilution buffer: 5% NFDM/TBST.

    • All lanes : Anti-Cdc37 (phospho S13) antibody [EPR4879] (ab108360) at 1/20000 dilution

      Lane 1 : HEK293 whole cell lysate - untreated
      Lane 2 : HEK293 whole cell lysate - treated with alkaline phosphatase

      Lysates/proteins at 10 µg per lane.

      Secondary
      Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

      Predicted band size : 44 kDa
      Observed band size : 44 kDa


      Exposure time : 3 minutes

      Blocking and dilution buffer: 5% NFDM/TBST.

    • Dot blot analysis of Cdc37 (pS13) phospho peptide (lane 1) and Cdc37 non-phospho peptide (lane 2) labelling Cdc37 (phospho S13) with ab108360 at a dilution of 1/1000. A peroxidase-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/2500).

      Blocking and dilution buffer: 5% NFDM/TBST.

      Exposure time: 3 minutes.

    • All lanes : Anti-Cdc37 (phospho S13) antibody [EPR4879] (ab108360)

      Lane 1 : Jurkat cell lysate (untreated)
      Lane 2 : Jurkat cell lysate (treated with Alkaline Phosphatase)

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 44 kDa

    Anti-Cdc37 (phospho S13) antibody [EPR4879] (ab108360) 使用論文

    ab108360 has not yet been referenced specifically in any publications.

    Product Wall

    Application Western blot
    Loading amount 30 µg
    Gel Running Conditions Reduced Denaturing (10)
    Sample Human Cell lysate - whole cell (HEK293)
    Specification HEK293
    Treatment CX-4945 25uM
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
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    投稿 Dec 09 2013

    Application Western blot
    Loading amount 20 µg
    Gel Running Conditions Reduced Denaturing (10%)
    Sample Human Cell lysate - whole cell (HEK293 cells)
    Specification HEK293 cells
    Treatment untreated or TDB treated (6h, 50 uM)
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Username

    Abcam user community

    Verified customer

    投稿 Oct 11 2013

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"