製品の概要

  • 製品名Anti-Cdc27 antibody
    Cdc27 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Cdc27
  • アプリケーション適用あり: WB, IPmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Dog, Xenopus laevis, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis
  • 免疫原

    Synthetic peptide corresponding to a region between residues 774 and 824 of human Cdc27 (NP_001247.2).

  • ポジティブ・コントロール
    • Whole cell lysates from HeLa and 293T cells.

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab72217 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/2000 - 1/10000. Detects a band of approximately 92 kDa (predicted molecular weight: 92 kDa).
IP Use at 2-5 µg/mg of lysate.

ターゲット情報

  • 機能Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains.
  • パスウェイProtein modification; protein ubiquitination.
  • 配列類似性Belongs to the APC3/CDC27 family.
    Contains 9 TPR repeats.
  • 翻訳後修飾Phosphorylated. Phosphorylation on Ser-426 and Thr-446 occurs specifically during mitosis.
  • 細胞内局在Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • ANAPC3 antibody
    • Anaphase Promoting Complex 3 antibody
    • Anaphase promoting complex protein 3 antibody
    • Anaphase Promoting Complex Subunit 3 antibody
    • Anaphase-promoting complex subunit 3 antibody
    • APC 3 antibody
    • APC3 antibody
    • Cdc 27 antibody
    • Cdc27 antibody
    • CDC27 homolog antibody
    • CDC27_HUMAN antibody
    • CDC27Hs antibody
    • Cell Division Cycle 27 antibody
    • Cell division cycle protein 27 homolog antibody
    • D0S1430E antibody
    • D17S978E antibody
    • H NUC antibody
    • H-NUC antibody
    • HNUC antibody
    • Nuc2 homolog antibody
    see all

Anti-Cdc27 antibody 画像

  • All lanes : Anti-Cdc27 antibody (ab72217) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg


    Predicted band size : 92 kDa
    Observed band size : 92 kDa
    Additional bands at : 55 kDa,60 kDa,68 kDa. We are unsure as to the identity of these extra bands.
  • Detection of Human Cdc27 by Immunoprecipitation from Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded) using ab72217 at 3 µg/mg lysate for IP (Lane 1) and at 1 µg/ml for subsequent WB detection. Lane 2 represents control IgG IP.

Anti-Cdc27 antibody (ab72217) 使用論文

ab72217 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"