The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2000 - 1/10000. Detects a band of approximately 92 kDa (predicted molecular weight: 92 kDa).
Use at 2-5 µg/mg of lysate.
Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains.
Protein modification; protein ubiquitination.
Belongs to the APC3/CDC27 family. Contains 9 TPR repeats.
Phosphorylated. Phosphorylation on Ser-426 and Thr-446 occurs specifically during mitosis.
All lanes : Anti-Cdc27 antibody (ab72217) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg Lane 2 : HeLa whole cell lysate at 15 µg Lane 3 : HeLa whole cell lysate at 5 µg Lane 4 : 293T whole cell lysate at 50 µg
Predicted band size: 92 kDa Observed band size: 92 kDa Additional bands at: 55 kDa, 60 kDa, 68 kDa. We are unsure as to the identity of these extra bands.
Immunoprecipitation - Cdc27 antibody (ab72217)
Detection of Human Cdc27 by Immunoprecipitation from Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded) using ab72217 at 3 µg/mg lysate for IP (Lane 1) and at 1 µg/ml for subsequent WB detection. Lane 2 represents control IgG IP.