製品の概要
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製品名Anti-Cdc27 antibody
Cdc27 一次抗体 製品一覧 -
製品の詳細Rabbit polyclonal to Cdc27
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由来種Rabbit
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アプリケーション適用あり: WB, IPmore details
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種交差性交差種: Human
交差が予測される動物種: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Xenopus laevis, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
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免疫原
Synthetic peptide corresponding to a region between residue 225 and 275 of human Cdc27 (NP_001247.2).
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ポジティブ･コントロール
- Whole cell lysate from HeLa and 293T cells.
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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バッファーPreservative: 0.09% Sodium Azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... -
精製度Immunogen affinity purified
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ポリ/モノポリクローナル
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アイソタイプIgG
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研究分野
関連製品
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Compatible Secondaries
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Positive Controls
アプリケーション
Our Abpromise guarantee covers the use of ab72214 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | 1/2000 - 1/10000. Predicted molecular weight: 92 kDa. | |
| IP | Use at 2-5 µg/mg of lysate. |
ターゲット情報
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機能Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains.
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パスウェイProtein modification; protein ubiquitination.
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配列類似性Belongs to the APC3/CDC27 family.
Contains 9 TPR repeats. -
翻訳後修飾Phosphorylated. Phosphorylation on Ser-426 and Thr-446 occurs specifically during mitosis.
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細胞内局在Nucleus.
- Information by UniProt
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参照データベース
- Entrez Gene: 540660 Cow
- Entrez Gene: 996 Human
- Entrez Gene: 217232 Mouse
- Entrez Gene: 360643 Rat
- Omim: 116946 Human
- SwissProt: A7Z061 Cow
- SwissProt: P30260 Human
- SwissProt: A2A6Q5 Mouse
see all -
別名
- ANAPC3 antibody
- Anaphase Promoting Complex 3 antibody
- Anaphase promoting complex protein 3 antibody
see all
画像
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Western blot - Cdc27 antibody (ab72214)All lanes : Anti-Cdc27 antibody (ab72214) at 0.04 µg/ml
Lane 1 : Whole cell lysate from HeLa cells at 50 µg
Lane 2 : Whole cell lysate from HeLa cells at 15 µg
Lane 3 : Whole cell lysate from HeLa cells at 5 µg
Lane 4 : Whole cell lysate from 293T cells at 50 µg
Predicted band size: 92 kDa
Observed band size: 100 kDa (why is the actual band size different from the predicted?)
Additional bands at: 45 kDa, 50 kDa, 70 kDa. We are unsure as to the identity of these extra bands. -
Immunoprecipitation - Cdc27 antibody (ab72214)Detection of Human Cdc27 by Immunoprecipitation in Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded), using ab72214 at 3 µg/mg lysate for IP and at 1 µg/ml for subsequent Western blot detection.
参考文献
This product has been referenced in:
- Ren YQ et al. MiR-27a modulates radiosensitivity of triple-negative breast cancer (TNBC) cells by targeting CDC27. Med Sci Monit 21:1297-303 (2015). WB ; Human . Read more (PubMed: 25943633) »
レビューと Q&A
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"