Cdc25C overexpression 293T lysate (whole cell) (ab94219)

製品の概要

  • 製品名Cdc25C overexpression 293T lysate (whole cell)
  • 製品の詳細
    Cdc25C overexpression lysate
  • 特記事項ab94219 is a 293T cell transfected lysate in which Human Cdc25C has been transiently over-expressed using a pCMV-Cdc25C plasmid. The lysate is provided in 1X Sample Buffer. Note: For more details about how the transfected lysate was prepared view preparation notes
  • アプリケーション適用あり: WBmore details

法規制情報

製品の特性

  • 背景Developmental stage: Expressed predominantly in G2 phase. Function: Functions as a dosage-dependent inducer in mitotic control. It is a tyrosine protein phosphatase required for progression of the cell cycle. It directly dephosphorylates CDK1 and activate its kinase activity. PTM: Phosphorylated by CHK1 on Ser-216. This phosphorylation creates a binding site for 14-3-3 protein and inhibits the phosphatase. Phosphorylated by PLK4. Similarity: Belongs to the MPI phosphatase family. Contains 1 rhodanese domain.

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab94219 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use at an assay dependent dilution.

Cdc25C overexpression 293T lysate (whole cell) 画像

  • ab94219 at 15µg/lane on an SDS-PAGE gel.
  • All lanes : Anti-Cdc25C antibody (ab66235) at 1/500 dilution

    Lane 1 : Cdc25C 293T Transfected Lysate - Positive Control (ab94219)
    Lane 2 : 293T non-transfected lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-mouse IgG (H and L) HRP conjugate at 1/2500 dilution

Cdc25C overexpression 293T lysate (whole cell) (ab94219) 使用論文

ab94219 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Questions for ab94219.
Please use the link above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"