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Recombinant fragment with tag: FRSNQRKMLN LLLERDTSFT VCPDVPRTPV GKFLGDSANL SILSGGTPKC CLDLSNLSSG EITATQLTTS ADLDETGHLD SSGLQEVHLA GMNHDQHLMK CSPAQLLCST, corresponding to amino acids 21-130 of Human Cdc25C
Our Abpromise guarantee covers the use of ab66235 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use at an assay dependent concentration.
The detection limit for recombinant tagged Cdc25C is approximately 0.1ng/ml as a capture antibody.
|IHC-P||Use a concentration of 6 µg/ml.|
|WB||Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 53 kDa (predicted molecular weight: 53 kDa).|
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: Cdc25C knockout HAP1 cell lysate (20 µg)
Lane 3: HeLa cell lysate (20 µg)
Lanes 1 - 3: Merged signal (red and green). Green - ab66235 observed at 58 kDa. Red - loading control, ab181602, observed at 37 kDa.
ab66235 was shown to recognize Cdc25C when Cdc25C knockout samples were used, along with additional cross-reactive bands. Wild-type and Cdc25C knockout samples were subjected to SDS-PAGE. ab66235 and ab181602 (loading control to GAPDH) were diluted 1 μg/mL and 1/10 000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed ab216772 and Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed ab216777 secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.
ab66235 has not yet been referenced specifically in any publications.